Summary
An immunoblotting method for C6 typing was developed. After isoelectric focusing in thin-layer polyacrylamide gel, C6 proteins were transferred passively to nitrocellulose and then detected by a two-step enzyme immunoassay. A population sample of northeastern Japanese was investigated using this method. Three common and four rare allotypes were observed. The allele frequencies estimated from 495 blood donors were as follows:C6 * A0.423, C6 * B0.510, C6 * B20.062, and rare alleles (91,M11, B4, andB5) 0.005. Three variants, 91,M11, andB5, were considered to be newly found. This method could be applicable to many other protein systems.
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Tokunaga, K., Yamamura, N. & Omoto, K. An immunoblotting technique for complement C6 typing: Three new variants. Jap J Human Genet 29, 415–419 (1984). https://doi.org/10.1007/BF01876499
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DOI: https://doi.org/10.1007/BF01876499
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