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Partial sequence of the tyrosine region of neocarzinostatin

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Zusammenfassung

Das antitumoraktive Protein-Antibiotikum Neocarzinostatin wurde mit Dithiothreit in flüssigem Ammoniak reduziert und mit Chloressigsäure alkyliert. Tryptische Spaltung des tetra-S-carboxymethylierten Proteins ergab 5 Fragmente. Die Sequenz von 25 Aminosäureresten im tyrosinhaltigen Fragment H3 wurde durchEdman-Abbau im automatisierten Sequenator ermittelt.

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Authors and Affiliations

  1. Children's Cancer Research Foundation and Dept. of Biological Chemistry, Harward Medical School, 02115, Boston, Massachussett, USA

    C. B. Glaser, H. Maeda, J. Meienhofer & H. D. Niall

  2. Endocrine Unit, Massachussetts General Hospital, 02114, Boston, Massachusetts, USA

    C. B. Glaser, H. Maeda, J. Meienhofer & H. D. Niall

Authors
  1. C. B. Glaser
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  2. H. Maeda
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  3. J. Meienhofer
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  4. H. D. Niall
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Additional information

This work was supported in part by Public Health Service Research Grants No. C-6516 from the National Cancer Institute and No. FR-05526 from the Division of Research Facilities and Resource, National Institutes of Health, to the Children's Cancer Research Foundation and by grants No. AM 04501 and No. AM 11794 from the National Institute of Arthritis and Metabolic Diseases to the Endocrine Unit, Massachusetts General Hospital.

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Glaser, C.B., Maeda, H., Meienhofer, J. et al. Partial sequence of the tyrosine region of neocarzinostatin. Experientia 28, 772–773 (1972). https://doi.org/10.1007/BF01923120

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