Résumé
L'ecdystérone, hormone de mue des insectes, a été extraite de homards femelles (Homarus americanus) venant de muer. Sa concentration moyenne est de 6μg/kg de homard vivant. L'assimilation de cholestérol (4-14C) par les homards femelles avant la mue et la biosynthèse d'ecdystérone à partir de ce précurseur est démontrée.
References
J. B. Sochasky, D. E. Aiken andN. H. F. Watson, Can. J. Zool.50, 993 (1972).
F. Hampshire andD. H. S. Horn, Chem. Commun.1966, 37.
A. Faux, D. H. S. Horn, E. J. Middleton, H. M. Fales andM. E. Lowe, Chem. Commun.,1969, 175.
D. S. King andJ. B. Siddall, Nature, Lond.221, 955 (1969).
E. Spaziani andS. B. Kater, Gen. comp. Endocr.20, 534 (1973).
D. A. Schooley andK. Nakanishi, inModern Methods of Steroid Analysis (Ed.E. Heftmann; Academic Press, New York, N.Y. 1973), p. 37.
N. Ikekawa, F. Hattori, J. Rubio-Lightbourn, H. Miyazaki, M. Ishibashi andC. Mori, J. Chromat. Science10, 233 (1972). —H. Miyazaki, M. Ishibashi andC. Mori, Analyt. Chem.45, 1164 (1973).
D.A. Schooley, G. Weiss andK. Nakanishi, Steroids19, 377 (1972).
D. H. S. Horn, inNaturally Occurring Insecticides (Eds.M. Jacobson andP. G. Crosby; Marcel Dekker, New York, N.Y. 1971), p. 333. —K. Nakanishi, XXIII vol. Int. Congr. Pure and Applied Chemistry (Butterworths, London 1971), vol. 3, p. 27.
The T. C. U. group has experienced a high degree of success in inducing the molting condition in lobsters by eye stalk ablation. Aritene, a form of microcrystalline collagen, was used as a hemostat (Aricon, Inc., Box 85, Fort Worth, Texas 76101, USA). Progress towards molt was followed byx-raying the gastroliths. Usually 30–45 days was required for an animal with no initial gastroliths to molt. For lobsters in the 400–500 g range a length of about 1.6 cm for the gastroliths indicated an imminent onset of molting.
M. N. Galbraith, D. H. S. Horn, E. J. Middleton andR. J. Hackney, Chem. Commun.1968, 83.
R. W. Flint, J. Fish. Res. Bd. Canada29, 1229 (1972).
D. S. King, Am. Zoologist12, 343 (1972).
This work.
T. Takemoto, S. Ogawa, N. Nishimoto andH. Hoffmeister, Z. Naturforsch.22b, 681 (1967).
P. Karlson andP. Schmialek, Hoppe-Seyler's Z. physiol. Chem.316, 83 (1959).
We thank ProfessorK. Nakanishi for valuable discussions, ProfessorsHorn andTakemoto for samples, and R. Beebe-Center for help in the initial stages of this work. Financial support by the Office of Sea Grant of the National Oceanic and Atmospheric Administration, U.S. Department of Commerce, the Research Corporation, the Petroleum Research Foundation of the American Chemical Society, the Robert A. Welch Foundation and the T.C.U. Research Foundation is gratefully acknowledged.
Author information
Authors and Affiliations
Additional information
Woods Hole Oceanographic Institution Contribution Number 3147.
Rights and permissions
About this article
Cite this article
Gagosian, R.B., Bourbonniere, R.A., Smith, W.B. et al. Lobster molting hormones: Isolation and biosynthesis of ecdysterone. Experientia 30, 723–724 (1974). https://doi.org/10.1007/BF01924149
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01924149