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Purification of galactose-binding phytoagglutinins and phytotoxin by affinity column chromatography using sepharose

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Zusammenfassung

Phytoagglutinine, welche Galaktose spezifisch binden, wurden mit Chromatographie an Sepharose gereinigt. Zwei Fraktionen ausRicinus communis-Agglitomom wiurden durch Gelfiltration mittels Bio-gel getrennt: die früh eluierte Fraktion zeigte starke Agglutinationsaktivität und schwache Toxizität, während die später eluierte Fraktion eine schwache Agglutinatinsaktivität bei starker Toxizität zeigt.

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Letiratur

  1. M. Tomita, T. Osawa, Y. Sakurai andT. Ukita, Int. J. Cancer,6, 283 (1970).

    Article  CAS  PubMed  Google Scholar 

  2. R. E. Pollack andM. M. Burger, Proc. natn. Acad. Sci. USA62, 1074 (1969).

    Article  CAS  Google Scholar 

  3. M. M. Burger, Nature, Lond.228, 512 (1970).

    Article  CAS  PubMed  Google Scholar 

  4. L.L. So andI. J. Goldstein, Biochim. biophys. Acta.165, 398 (1968).

    Article  CAS  PubMed  Google Scholar 

  5. A. Moriwaki, Gann54, 323 (1963).

    CAS  Google Scholar 

  6. J. W. Parker, A. Steiner andR. J. Lukes, Experientia25, 187 (1969).

    Article  CAS  PubMed  Google Scholar 

  7. K. O. Lloyd, Arch. Biochem. Biophys.137, 460 (1970).

    Article  CAS  PubMed  Google Scholar 

  8. E. Waldschmidt-Leitz andL. Keller Z. physiol. Chem.351, 990 (1970).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan

    M. Tomita, T. Kurokawa, K. Onozaki, N. Ichiki, T. Osawa & T. Ukita

Authors
  1. M. Tomita
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  2. T. Kurokawa
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  3. K. Onozaki
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  4. N. Ichiki
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  5. T. Osawa
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  6. T. Ukita
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Additional information

We thank Dr. Y.Sakurai, Department of Chemotherapy, Cancer Istitute, for his constant interest and encouragement during the execution of this work.

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Tomita, M., Kurokawa, T., Onozaki, K. et al. Purification of galactose-binding phytoagglutinins and phytotoxin by affinity column chromatography using sepharose. Experientia 28, 84–85 (1972). https://doi.org/10.1007/BF01928278

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  • DOI: https://doi.org/10.1007/BF01928278

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