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An improved test for Africanized honeybee mitochondrial DNA

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Abstract

Mitochondrial DNA derived fromApis mellifera scutellata, the ancestor of the Africanized bees of the New World, lacks aBglII restriction site found in other types of honeybee1,2. We present primers allowing amplification of a 485-bp section of the cytochrome b gene containing this site, using the polymerase chain reaction. Digestion of the amplifiel product withBglII yields contrasting patterns between Africanized and other honeybees.

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Authors and Affiliations

  1. Department of Genetics & Human Variation, La Trobe University, 3083, Bundoora, V., Australia

    Y. C. Crozier, S. Koulianos & R. H. Crozier

Authors
  1. Y. C. Crozier
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  2. S. Koulianos
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  3. R. H. Crozier
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Crozier, Y.C., Koulianos, S. & Crozier, R.H. An improved test for Africanized honeybee mitochondrial DNA. Experientia 47, 968–969 (1991). https://doi.org/10.1007/BF01929894

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  • DOI: https://doi.org/10.1007/BF01929894

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