Summary
A commercially available protein-assay, based on the reaction between bicinchoninic acid (4,4′-dicarboxy-2,2′-biquinoline) and copper was adapted for use in microtiter plates. The assay is sensitive between 10 μm/ml and several mg/ml, and it uses only 10 μl of sample material. The results obtained with the method were in accordance with other protein determination methods. The assay was shown to be reproducible, reliable and insensitive to non-ionic detergents.
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Literature
Pierce Product Information, No 23225.
Wang, C.-S., and Smith, R. L., Analyt. Biochem.63 (1975) 414.
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Acknowledgments. The authors are grateful to Miss Barbara Moser who skillfully performed a number of these experiments. This research was supported by the Swiss National Science Foundation, Grant No 3.300–0.82, to U.B.
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Sorensen, K., Brodbeck, U. A sensitive protein assay method using micro-titer plates. Experientia 42, 161–162 (1986). https://doi.org/10.1007/BF01952446
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DOI: https://doi.org/10.1007/BF01952446