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Kinetics of lymphocyte division in blood cultures studied with the BrdU-giemsa technique

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Summary

Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 μg/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.

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Authors and Affiliations

  1. Instituto Multidisciplinario de Biologia Celular (IMBICE), Calle 526 entre 10 y 11, La Plata, Argentina

    N. O. Bianchi & Esther A. Lezana

Authors
  1. N. O. Bianchi
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  2. Esther A. Lezana
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Additional information

This work was supported by grants from the International Atomic Energy Agency, the ‘Consejo Nacional de Investigaciones Cientificas’ and the ‘Comisión de Investigaciones Cientificas’.

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Bianchi, N.O., Lezana, E.A. Kinetics of lymphocyte division in blood cultures studied with the BrdU-giemsa technique. Experientia 32, 1257–1259 (1976). https://doi.org/10.1007/BF01953080

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  • DOI: https://doi.org/10.1007/BF01953080

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