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Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia

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Substance P and choline acetyltransferase have been localised in a small proportion of endothelial cells of rat coronary arteries using electron microscopic immunocytochemistry. During a hypoxic period of 1 min, coronary vasodilatation was produced in the Langendorff heart preparation and increased levels of substance P and acetylcholine were released into the perfusate. The possibility that these substances are released from endothelial cells during hypoxia and contribute to the hyperaemic response is discussed.

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Authors and Affiliations

  1. Department of Anatomy and Developmental Biology, University College London, Gower Street, WC1E 6BT, London

    P. Milner, V. Ralevic, A. M. Hopwood, J. Lincoln, K. A. Kirkpatrick & G. Burnstock

  2. First Department of Anatomy, Semmelweiss University Medical School, Tüzoltó u 58, H-1450, Budapest, Hungary

    E. Fehér

Authors
  1. P. Milner
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  2. V. Ralevic
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  3. A. M. Hopwood
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  4. E. Fehér
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  5. J. Lincoln
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  6. K. A. Kirkpatrick
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  7. G. Burnstock
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Milner, P., Ralevic, V., Hopwood, A.M. et al. Ultrastructural localisation of substance P and choline acetyltransferase in endothelial cells of rat coronary artery and release of substance P and acetylcholine during hypoxia. Experientia 45, 121–125 (1989). https://doi.org/10.1007/BF01954843

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  • DOI: https://doi.org/10.1007/BF01954843

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