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Quantification of Globodera rostochiensis and G. pallida in mixed populations using species-specific thermostable proteins

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Abstract

A method has been developed to quantify species ratios in mixed populations. The method is based on the separation of species-specific thermostable proteins by SDS-PAGE. Densitometric analyses of the 17 kD protein ofGlobodera pallida and the 18 kD protein ofG. rostochiensis revealed a high correlation (R 2=0.93) with the species ratio in the mixed samples. Within the limits of 10 to 90% of each species, one can estimate with 95% reliability the species composition with 3 to 6% deviation.

Samenvatting

Een methode is ontwikkeld om de samenstelling van soortenmengsels vanGlobodera rostochiensis enG. pallida te kwantificeren. Bij deze methode wordt gebruik gemaakt van de soort-specifieke thermostabiele eiwitten die met behulp van SDS-PAGE gescheiden worden. De kleurintensiteit van het 17 kD eiwit vanG. pallida en het 18 kD eiwit vanG. rostochiensis is per gel-laan bepaald m.b.v. een densitometer en heeft een lineair verband met de soortsverhouding in de mengsels (R 2=0.93). Binnen het bereik van 10 tot 90% van elke soort kan men met deze ijklijn met 95% betrouwbaarheid de soortsamenstelling bepalen op 3 tot 6% nauwkeurig.

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Authors and Affiliations

  1. Research Institute for Plant Protection, P.O. Box 9060, 6700 GW, Wageningen, the Netherlands

    L. J. M. F. Den Nijs & C. A. M. Lock

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  1. L. J. M. F. Den Nijs
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  2. C. A. M. Lock
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Den Nijs, L.J.M.F., Lock, C.A.M. Quantification of Globodera rostochiensis and G. pallida in mixed populations using species-specific thermostable proteins. Netherlands Journal of Plant Pathology 96, 179–186 (1990). https://doi.org/10.1007/BF01974255

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