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High resolution isoelectric focusing of juvenile hormone esterase activity from the hemolymph ofTrichoplusia ni (Hübner)

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  • Biochemistry and Biophysics, Metabolism, Neurobiology, Pharmacology
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Summary

Juvenile hormone esterase (JHE) activity from the hemolymph of larvalTrichoplusia ni was analyzed by two different isoelectric focusing (IEF) methodologies. Use of techniques capable of progressively higher resolution split ultimately what appeared at lower resolution to be a single peak into two discrete peaks of JHE activity (pI 5.5 and 5.3). Neither peak was a degradation artifact of the other caused by conditions of IEF.

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Authors and Affiliations

  1. Department of Entomology, University of Kentucky, 40546-0091, Lexington, Kentucky, USA

    D. Jones, G. Jones, M. Rudnicka, A. J. Click & S. C. Sreekrishna

Authors
  1. D. Jones
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  2. G. Jones
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  3. M. Rudnicka
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  4. A. J. Click
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  5. S. C. Sreekrishna
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Additional information

We thank Drs Kenneth V. Yeargan and Gerald L. Nordin for their review of the manuscript. This study was supported, in part, by NIH Grant GM33995-01 and is published with the approval of the Director as paper no. 85-7-168 in connection with a project of the Kentucky Agricultural Experiment station.

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Jones, D., Jones, G., Rudnicka, M. et al. High resolution isoelectric focusing of juvenile hormone esterase activity from the hemolymph ofTrichoplusia ni (Hübner). Experientia 42, 45–47 (1986). https://doi.org/10.1007/BF01975888

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  • DOI: https://doi.org/10.1007/BF01975888

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