Summary
When HeLa cells, chicken embryo fibroblasts, sarcoma Galliera cells, Friend leukemia virus transformed cells and human lymphocytes are cultured in a hypergravitational field (e.g. 10×g) proliferation rate is increased by 20–30%, whereas glucose consumption per cell is lower than at 1×g. Tracking of cell movements on gold-coated substrates reveals that cell migration is hindered at high-g. These findings suggest that under gravitational stress the cell is either capable of shifting to other metabolic pathways and/or consumes less energy at high-g than at 1×g. This work describes ground-based investigations related to experiments to be performed on future Spacelab missions.
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We wish to thank P. Bislin for her excellent technical assistance, J.F. Conscience for providing us with FBU 3b cells and P. Pippia for SGS-3 cells and for their useful advice, K. Bienz and W. Briegleb for reviewing the manuscript and B. Huber for manufacturing the high-g centrifuge. This work was supported by the Swiss National Science Foundation, Berne, grant No. 3.034–0.81.
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Tschopp, A., Cogoli, A. Hypergravity promotes cell proliferation. Experientia 39, 1323–1329 (1983). https://doi.org/10.1007/BF01990088
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DOI: https://doi.org/10.1007/BF01990088