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A comparison of rat peritoneal mast cells purified using Percoll and Path-O-Cyte 4

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  • Histamine Release
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Abstract

Rat peritoneal mast cells (RPMC) purified through Percoll and a commercially available 35% bovine serum albumin (BSA) solution (Path-O-Cyte 4) are compared over a range of criteria with mixed peritoneal cell suspensions. Both the purity and spontaneous histamine release were slightly better in RPMC isolated through Percoll and histamine recovery was considerably greater using this method as opposed to Path-O-Cyte 4. The responsitivity of cells isolated by either method to compound 48/80 and ionomycin was comparable with that seen in non-purified RPMC. However, the histamine release induced by anti-rat IgE and concanavalin A was reduced in the purified cells especially in those isolated through BSA. Histamine release induced by IgE-directed ligands was potently inhibited by theophylline, isobutyl methyl xanthine, disodium cromoglycate and nedocromil sodium in both non-purified and Percoll-purified RPMC. In total, this study has shown that highly purified and viable RPMC can be obtained through Percoll purification. This method was seen to be generally superior to that of Path-O-Cyte 4.

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References

  1. A. R. Johnson and N. C. Moran,Comparison of several methods for isolation of rat peritoneal mast cells. Proc. Soc. exp. Biol. Med.123, 886–888 (1966).

    PubMed  Google Scholar 

  2. W. M. Purcell, D. L. Cohen and T. H. P. Hanahoe,Contribution of post-secretory mechanisms to the observed pattern of histamine and 5-hydroxytryptamine secretion from peritoneal rat mast cells in response to compound 48/80. Int. Archs. Allergy appl. Immunol.90, 387–394 (1989).

    Google Scholar 

  3. T. J. Sullivan, K. L. Parker, W. Stenson and C. W. Parker,Modulation of cyclic AMP in purified rat mast cells. 1. responses to pharmacologic, metabolic and physical stimuli. J. Immunol.114, 1473–1479 (1975).

    PubMed  Google Scholar 

  4. J. R. White and F. L. Pearce,Characteristics of histamine secretion from rat peritoneal mast cells sensitized to the nematode Nippostrongylus brasiliensis. Immunology46, 353–359 (1982).

    PubMed  Google Scholar 

  5. H. S. Gilbert and L. Ornstein,Basophil counting with a new method using Alcian blue. Blood46, 279–286 (1975).

    PubMed  Google Scholar 

  6. L. Enerbäck and I. Svensson,Isolation of rat peritoneal mast cells by centrifugation on density gradients of Percoll. J. immunol. Methods39, 135–145 (1980).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University College London, 20 Gordon Street, WC1H OAJ, London, UK

    G. A. Mackay & F. L. Pearce

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  1. G. A. Mackay
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  2. F. L. Pearce
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Mackay, G.A., Pearce, F.L. A comparison of rat peritoneal mast cells purified using Percoll and Path-O-Cyte 4. Agents and Actions 36 (Suppl 2), C315–C317 (1992). https://doi.org/10.1007/BF01997361

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  • DOI: https://doi.org/10.1007/BF01997361

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