Abstract
The ability of bacteria to attach to surfaces has been recognized as an important phenomenon, particularly for pathogenic organisms that utilize this capacity to initiate disease. The present investigation was undertaken to determine whether indigenous urogenital bacteria, lactobacilli, colonized prosthetic devices in vivo and in vitro and attached to specific polymer surfaces in vitro. Polyethylene intrauterine devices (IUDs) in place for 2 years were removed from six women who were asymptomatic and free of signs of cervical or uterine infection. Lactobacilli were found attached to the IUDs, as determined by culture, and fluorescent antibody and acridine orange staining techniques. This demonstrated that bacterial biofilms consisting of indigenous bacteria can occur on prosthetic devices without inducing a symptomatic infection. In vitro studies were then undertaken with well-documented lactobacilli strainsL. acidophilus T-13,L. casei GR-1, GR-2, and RC-17, andL. fermentum A-60. These organisms were found to adhere to IUDs and urinary catheters within 24 hours. A quantitative assay was designed to examine the mechanisms of adhesion ofL. acidophilus T-13 to specific polymer surfaces that are commonly used as prosthetic devices. The lactobacilli adhered optimally to fluorinated ethylene propylene when 108 bacteria were incubated for 9 hours at 37°C in phosphate buffered saline, pH 7.1. Additional experiments verified that the lactobacilli adhered to polyethyleneterephthalate, polystyrene, and sulfonated polystyrene and to silkolatex catheter material. There was a linear relationship found between polymer hydrophobicity and bacterial adherence. These results demonstrate that lactobacilli bind to various surfaces in vivo and in vitro, and that the nature of the substratum can affect the colonization.
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Absolom DR, Lamberti FV, Policova Z, Zingg W, van Oss CJ, Neumann AW (1983) Surface thermodynamics of bacterial adhesion. Appl Environ Microbiol 46:90–97
Absolom DR, Neumann AW, Zingg W, van Oss CJ (1979) Thermodynamic studies of cellular adhesion. Trans Am Soc Artif Intern Org 25:152–156
Bruce AW, Sira SS, Clark AF, Awad SA (1974) The problem of catheter encrustation. CMAJ 111:238–241
Bruce AW, Reid G (1988) Intravaginial instillation of Lactobacilli for prevention of urinary tract infections. Can J Microbiol 34:339–343
Chan RCY, Bruce AW, Reid G (1984) Adherence of cervical, vaginal and distal urethral normal microbial flora to human uroepithelial cells and the blockage of adherence of uropathogens by competitive exclusion. J Urol 131:596–601
Chan RCY, Reid G, Irvin RT, Bruce AW, Costerton JW (1985) Competitive exclusion of uropathogens from uroepithelial cells byLactobacillus whole cells and cell wall fragments. Infect Immun 47:84–89
Cook R, Tannock G, Meech R (1984) The vaginal microflora and nonspecific vaginitis. Proc University of Otago Medical School 63:10–11
Costerton JW, Marrie TJ, Cheng K-J (1985) Phenomena of bacterial adhesion. In: Savage DC and Fletcher M (eds) Bacterial adhesion: mechanisms and physiological significance. Plenum Press, New York, pp 3–43
Garden AS, Reid G, Benzie RJ (1985) Chorionic villus sampling. Lancet 1:1270
Gibbons RJ, van Haute J (1975) Bacterial adherence in oral microbial ecology. Ann Rev Microbiol 29:19–44
Ladd TI, Schimiel D, Nickel JC, Costerton JW (1985) Rapid method for detection of adherent bacteria on Foley urinary catheters. J Clin Microbiol 21:1004–1006
Marrie TJ, Costerton JW (1983) Bacterial colonization of intrauterine contraceptive devices. Am J Obstet Gynecol 146:384–394
Marrie TJ, Costerton JW (1983) A scanning electron microscopic study of the adherence of uropathogens to a plastic surface. Appl Environ Microbiol 45:1018–1024
Marrie TJ, Swantee CA, Hartlen M (1980) Aerobic and anaerobic urethral flora of healthy females in various physiological age groups and of females with urinary tract infections. J Clin Microbiol 11:654–659
Reid G, Brooks HJL (1985) A fluorescent antibody staining technique to detect bacterial adherence to urinary tract epithelial cells. Stain Technol 60:211–217
Reid G, Chan RCY, Bruce AW, Costerton JW (1985) Prevention of urinary tract infection in rats using an indigenous strain ofLactobacillus. Infect Immun 49:320–324
Reid G, Cook RL, Angotti R, Andre JK, Bruce AW (1987) Bacterial interference and the role ofLactobacillus spp. in preventing recurrent urinary tract infection. In: Murakami K, Kitagawa T, Yabuta K, Sakai T (eds) Recent advances in pediatric nephrology. Excerpta Medica, Amsterdam, ICS 733:265–270
Reid G, Cook RL, Bruce AW (1987) Examination of strains of lactobacilli for properties which may influence bacterial interference in the urinary tract. J Urol 138:330–335
Reid G, Sobel JD (1987) Bacterial adherence in the pathogenesis of urinary tract infection: a review. Rev Infect Dis 9:470–487
Reid G, Cook RL, Hagberg L, Bruce AW (1988) Lactobacilli as competitive colonizers of the urinary tract. Proc 4th Intl Meeting on Pyelonephritis. In: Host-Parasite Interactions in Urinary Tract Infections. University of Chicago Press.
Savage DC (1985) Effects on host animals of bacteria adhering to epithelial surfaces. In: Savage DC, Fletcher M (eds) Bacterial adhesion: mechanisms and physiological significance. Plenum Press, New York, pp 437–463
Sharpe E (1981) The genusLactobacillus. In: Starr MP, Stolp H, Truper HG, Balows A, Schlegel HG (eds) The prokaryotes. A handbook on habitats, isolation, and identification of bacteria. Springer-Verlag, New York, pp 1653–1679
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Reid, G., Hawthorn, L.A., Mandatori, R. et al. Adhesion of lactobacilli to polymer surfaces in vivo and in vitro. Microb Ecol 16, 241–251 (1988). https://doi.org/10.1007/BF02011698
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DOI: https://doi.org/10.1007/BF02011698