Abstract
Cultures of the soil inhabiting fungusPythium spec. were inoculated in vitro with tobacco mosaic virus. Virus could be demonstrated in the mycelia from 4 days on after inoculation. In 15 days old cultures the virus concentration in the mycelium was higher than in the liquid culture medium. It is not yet clear whether the virus only accumulates, or also multiplies in the mycelium. After growth on solid medium infected mycelia still contained virus indicating that the virus is able to persist and possibly also to multiply in the hyphae.
Samenvatting
Cultures van de bodemschimmelPythium spec. werden in vitro geïnoculeerd met tabaksmozaïekvirus. In enkele gevallen kon al 4 dagen na inoculatie virus in mycelium worden aangetoond. In 15 dagen oude cultures bevatte het mycelium meer virus dan de cultuurvloeistof. Het is nog niet bekend of het virus zich slechts ophoopt of zich ook vermeerdert in het mycelium. Na groei op een agarmedium gedurende 7 dagen bevatte het mycelium nog virus, wat er op zou wijzen dat het virus in staat is zich te handhaven in de hyfen en zich daarin wellicht ook te vermeerderen.
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Hendrina Brants, D. Tobacco mosaic virus in Pythium spec. Netherlands Journal of Plant Pathology 75, 296–299 (1969). https://doi.org/10.1007/BF02015491
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DOI: https://doi.org/10.1007/BF02015491