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Simple rapid identification of Candida albicans with emphasis on the differentiation between Candida albicans and Candida stellatoidea

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Summary

Subcultures ofC. albicans, made from Sabouraud agar, grown at room temperature for 48 hours, were inoculated into a 10 times saline dilution of Sabouraud liquid medium and left in the incubator for 45–60 minutes at 37° C, transferred to corn meal agar plates and incubated at 37° C for 18–24 hours.

Small portions of the surface agar containing the yeasts from these plates were pressed under cover glasses and examined under the oil immersion lens.

Under these conditions,C. albicans cultures were observed to produce only yeast-like cells, whereasC. stellatoidea cultures contained predominantly abundant, long, thin mycelia.

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References

  • Buckley, H. R. &Uden, N. Van. 1963. The Identification ofCandida Albicans Within Two Hours by the Use of an Egg-White Slide Preparation. Sabouraudia2: 205–208.

    Google Scholar 

  • Hill, D. W. &Gebhart, L. P. 1956. Morphological Transformation ofCandida Albicans in Tissue of Mice. Proc. Soc. exp. Biol. Med.92: 640–644.

    PubMed  Google Scholar 

  • Hurley, R. 1965. The Pathogenicity ofCandida Stellatoidea. J. Path. Bact.90, 1: 351–354.

    PubMed  Google Scholar 

  • Jones, C. P. &Peck, R. L. 1940. A Green Pigment fromCandida Stellatoidea andCandida Albicans. J. Bact.39: 605–608.

    Google Scholar 

  • Kaminsky, D. &Quinlan, L. 1963. A simple Inexpensive Method for Rapid Identification ofCandida Albicans. Amer. J. clin. Path.40: 143–145.

    Google Scholar 

  • Kelly, J. P. &Funigiello, F. 1959.Candida Albicans; A Study of Media Designed to Promote Chlamydospore Production. J. Lab. clin. Med.53: 807–809.

    PubMed  Google Scholar 

  • Landau, J. W., Dabrowa, N. &Newcomer, V. D. 1965. The Rapid Formation in Serum of Filaments byCandida Albicans. J. invest. Derm.44, 3: 171–179.

    PubMed  Google Scholar 

  • Mackenzie, D. W. R. 1962. Serum Tube Identification ofCandida Albicans. J. clin. Path.15: 563–565.

    Google Scholar 

  • Mackinnon, J. E. 1960. Dissociation inCandida Albicans. J. infect. Dis.66: 59–77.

    Google Scholar 

  • Martin, D. M., Jones, C. P., Yao, K. F. &Lee, L. E. Jr., 1937. Practical Classification of the Monilias. J. Bact.34: 99–129.

    Google Scholar 

  • Martin, D. S. &Jones, C. P. 1940. Further Studies on the Practical Classification of the Monilias. J. Bact.34: 609–630.

    Google Scholar 

  • Mickle, W. A. &Jones, C. P. 1940. Dissociation ofCandida Albicans by Lithium Chloride and Immune Serum. J. Bact.39: 638–640.

    Google Scholar 

  • Nickerson, W. J. 1953. Reduction of Inorganic Substances by Yeast; Extracellular Reduction of Sulfite by Species ofCandida Albicans. J. infect. Dis.93: 43–56.

    PubMed  Google Scholar 

  • Nickerson, W. J. &Mankowski, Z. 1953. Polysaccharide Medium of Known Composition Favoring Chlamydospore Formation inCandida Albicans. J. infect. Dis.92: 20–25.

    PubMed  Google Scholar 

  • Scherr, G. H. &Weaver, R. H., 1953. The Dimorphism Phenomenon in Yeast. Bact. Rev.17: 51–92.

    PubMed  Google Scholar 

  • Taschdjian, C. L., Burchall, J. J. &Kozinn, P. J. 1960. Rapid Identification ofCandida Albicans by Filamentation on Serum and Serum Substitutes. A.M.A.J. Dis. Child.99: 212–215.

    Google Scholar 

  • Walker &Huppert, M. 1960. Corn Meal Tween Agar: An Improved Medium for the Identification ofCandida Albicans. Amer. J. clin. Path.33: 190–194.

    Google Scholar 

  • Widra, A. 1957. An improved Fermentation Method for Rapid Identification ofCandida Species. J. infect. Dis.,100: 70–73.

    PubMed  Google Scholar 

  • Winston, S. &Murray, T. J. 1956. Virulence Enhancement of a Filamentous Strain ofCandida Albicans After Growth on Media Containing Cystein. J. Bact.71: 738.

    PubMed  Google Scholar 

  • Winter, W. D. &Foley, G. E. 1955. Failure ofCandida Stellatoidea to Qualify as Species. J. infect. Dis.97, 227–230.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Clinical Pathology (Section of Bacteriology) Deer Lodge Hospital, D.V.A. Winnipeg 12, Manitoba

    T. Kamaya M. D.

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  1. T. Kamaya M. D.
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Kamaya, T. Simple rapid identification of Candida albicans with emphasis on the differentiation between Candida albicans and Candida stellatoidea. Mycopathologia et Mycologia Applicata 35, 105–112 (1968). https://doi.org/10.1007/BF02049573

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