Summary
The sera from normal subjects gave negative results with the following antigens used in the complement-fixation tests: 1) polysaccharide prepared according toFava Netto's technic; 2) a filtrate of shaked cultures followingAjello et al.'s technic; 3) an aqueous extract of mechanically disrupted yeast cells ofP. brasiliensis.
The sera from patients of S.A. blastomycosis gave positive c.f. tests with the three antigens with titer ranging from 1/20 to 1/5, 160. Antigen No 2 gave in 11/18 cases higher titers than the other antigens.
Immunodiffusion tests gave positive results with the antigen no 2. The sera from 10 cases of histoplasmosis gave cross reactions with the antigen No 3, in 5/10 cases with the antigen No 2 and in not any case with the antigen No 1.
Similar content being viewed by others
Bibliografía
Fava Netto, C. 1955. Estudos cuantitativos sobre a fixação do complemento na blastomicose Sulamericana com antigeno polisacaridico Tese. Medicina, São Paulo.
Ajello, L., Walls, K., Moore, J. C. &Falcone, R. 1959. Rapid production of complement fixation antigens for systemic mycotic diseases. J. Bact.77 6: 753–756.
Oster, A. C., Strauss, J. H. &Mayer, M. M. 1952. Diagnostic complement fixation. I. A method. Amer. J. Syph.36 2, 140–153.
Restrepo, M. A. 1966. La prueba de inmunodifusión en el diagnóstico de la Paracoccidioidomicosis. Sabouraudia,4 (4), 223–230.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Negroni, R., Negroni, P. Antígenos del paracoccidioides brasiliensis para las Reacciones serológicas. Mycopathologia et Mycologia Applicata 34, 285–288 (1968). https://doi.org/10.1007/BF02051852
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02051852