Summary
The cotyledon ofPhaseolus vulgaris L. comprises four tissues: epidermis, abaxial hypodermis, storage parenchyma, and procambium. A complex intercellular space system is present throughout the storage tissue and comprises about 16% of the cotyledon volume. All the cells contain protein bodies, and the hypodermis and storage parenchyma also contain starch grains. The epidermal cells are at the 2 C level of DNA, those of the hypodermis at the 4 C level, and the storage cells vary from 8 C to 32 C. During germination stomata differentiate in the epidermis. Reserve mobilization begins in the cells furthest from the epidermis and from the vascular tissue. Protein is removed from these cells with little or no coalescence of protein bodies. The DNA content of the nuclei decreases. The cell walls swell and then decrease in thickness as material is removed. Finally the nuclei and cytoplasm disappear and the cells collapse. In the cells near vascular bundles the protein bodies coalesce before losing their protein. The DNA content of the nuclei declines but nuclei and cytoplasm are still present at abscission. These cells do not collapse. Cytoplasmic RNA content is highest near the abaxial surface. Most of the RNA is removed during the first three days of germination.
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References
Bain, J. M., andF. V. Mercer, 1966: Subcellular organization of the cotyledons in germinating seeds and seedlings ofPisum sativum L. Aust. J. biol. Sci.19, 69–84.
Barka, T., andP. J. Anderson, 1963: Histochemistry: theory, practice, and bibliography. New York: Harper and Row.
Barker, G. R., andT. Douglas, 1960: Function of ribonuclease in germinating peas. Nature188, 943–944.
Briarty, L. G., D. A. Coult, andD. Boulter, 1970: Protein bodies of germinating seeds ofVicia faba. Changes in fine structure and biochemistry. J. Exp. Bot.21, 513–524.
Feder, N., andT. P. O'Brien, 1968: Plant microtechnique: some principles and new methods. Amer. J. Bot.55, 123–144.
Flinn, A. M., 1969: A nutritional study of fruit maturation inPisum arvense L. Ph. D. Thesis, The Queen's University, Belfast.
Gepstain, S., andI. Ilan, 1970: A promotive action of kinetin on amylase activity in cotyledons ofPhaseolus vulgaris. Pl. Cell. Physiol. (Tokyo)11, 819–822.
Horner, H. T., andH. J. Arnott, 1965: A histochemical and ultrastructural study ofYucca seed proteins. Amer. J. Bot.52, 1027–1038.
Horner, H. T., andH. J. Arnott, 1966: A histochemical and ultrastructural study of pre- and postgerminatedYucca seeds. Bot. Gaz.127, 48–64.
Jensen, W. A., 1962: Botanical histochemistry: principles and practice. San Francisco: Freeman.
MacLeod, A. M., andR. Sandie, 1961: Cell wall metabolism. I. Hemicelluloses ofBromus seeds. New Phytol.60, 117–128.
Martos, L., 1956: The nucleic acids of leguminous cotyledons. Naturwissenschaften43, 399–400.
Meier, H., 1958: On the structure of cell walls and cell wall mannans from ivory nuts and from dates. Biochim. biophys. Acta28, 229–240.
Meyer, A. M., andA. Poljakoff-Mayber, 1963: The germination of seeds. London: Pergamon Press.
Moss, G. I., 1967: A cytochemical study of DNA, RNA, and protein in the developing maize anther. I. Methods. Ann. Bot.31, 545–553.
Olsson, R., andD. Boulter, 1968: Nucleic acid metabolism ofVicia faba during germination and growth. Physiologia Pl.21, 428–434.
Oota, Y., andS. Osawa, 1954: Migration of “storage RNA” from cotyledon into growing organs of bean seed embryos. Experientia10, 254–256.
Öpik, H., 1966: Changes in cell fine structure in the cotyledons ofPhaseolus vulgaris L. during germination. J. Exp. Bot.17, 427–439.
—, andE. W. Simon, 1963: Water content and respiration rate of bean cotyledons. J. Exp. Bot.14, 299–310.
Smith, D. L., 1971: Nuclear changes in the cotyledons ofPisum arvense L. during germination. Ann. Bot.35, 511–521.
—, andA. M. Flinn, 1967: Histology and histochemistry of the cotyledons ofPisum arvense L. during germination. Planta (Berl.)74, 72–85.
Tombs, M. P., 1967: Protein bodies in the soybean. Pl. Physiol. (Lancaster)42, 797–813.
Varner, J. E., L. V. Balce, andR. C. Huang, 1963: Senescence of cotyledons of germinating peas. Influence of axis tissue. Pl. Physiol. (Lancaster)38, 88–92.
Walbot, V., 1971: RNA metabolism during embryo development and germination ofPhaseolus vulgaris. Develop. Biol.26, 369–379.
Weibel, E. R., andH. Elias, 1967: Introduction to sterologic principles. In: Quantitative methods of morphology (E. R. Weibel, andH. Elias, eds.). Berlin-Heidelberg-New York: Springer-Verlag.
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Smith, D.L. A histological and histochemical study of the cotyledons ofPhaseolus vulgaris L. during germination. Protoplasma 79, 41–57 (1974). https://doi.org/10.1007/BF02055782
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DOI: https://doi.org/10.1007/BF02055782