Skip to main content
SpringerLink
Log in

Uranium speciation in biological medium by means of capillary electrophoresis and time-resolved laser-induced fluorescence

  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

The study of the chemical behavior of uranium in biological medium is still of great interest. By the use of capillary electrophoresis (CE) and time-resolved laser-induced fluorescence (TRLIF), it is possible to characterize the different complexes of uranium. Hence, CE, using the isoelectric focusing mode (CIEF), allows for the separation of the different complexes as a function of their isoelectric points (pI) and TRLIF as a speciation method leads to the identification at very low level of different uranyl complexes by temporal resolution and spectral deconvolution. Results obtained on various inorganic chemical systems (phosphate, bicarbonate) together with biological systems (citrate, transferrin) will be presented and discussed. The complexation between uranium and human transferrin has been pointed out through CIEF.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. Voetglin, H. C. Hodge, Pharmacology and Toxicology of Uranium Compounds, McGraw-Hill Book Company, New York, 1953.

    Google Scholar 

  2. C. Pasquier, M. Bourguignon, CRSSA 76 Internal Report, 6 (1977) 149.

    Google Scholar 

  3. S. Chevari, D. Likhner, Med. Radiol., 13, 8 (1968) 53.

    Google Scholar 

  4. C. Moulin, P. Decambox, V. Moulin, J. G. Decaillon, Anal. Chem., 67 (1995) 348.

    Google Scholar 

  5. L. Couston, D. Pouyat, C. Moulin, P. Decambox, Appl. Spectrosc., 49, 3 (1995) 349.

    Google Scholar 

  6. C. Moulin, P. Decambox, P. Mauchien, V. Moulin, M. Theyssier, Radiochim. Acta, 52/53 (1991) 119.7.

    Google Scholar 

  7. V. Moulin, J. Tits, C. Moulin, P. Decambox, P. Mauchien, O. De Ruty, Radiochim. Acta, 58/59 (1992) 121.

    Google Scholar 

  8. C. Moulin, P. Reiller, C. Beaucaire, D. Lemordant, J. Coll. Int. Science, 157 (1993) 411.

    Google Scholar 

  9. W. Stevens, F. Bruenger, G. N. Taylor, The distribution and retention of hexavalen233U in the beagle, in: Actinides in Man and Animals,M. D. Wrenn (Ed.) 1979.

  10. F. Kilar, S. Hjerten, Electrophoresis, 10 (1989) 23.

    PubMed  Google Scholar 

  11. E. N. Baker, Adv. Inorg. Chem., 41 (1994) 389.

    Google Scholar 

  12. S. Hjerten, J. Chromatogr., 347 (1985) 191.

    Google Scholar 

  13. I. Grenthe, J. Fuger, R. Lemire, R. J. Muller, C. Nguyen-Trung, H. Wanner, Chemical Themodynamics of Uranium. OECD Nuclear Energy Agency, North Holland, Amsterdam-London-New York-Tokyo, 1992.

    Google Scholar 

  14. I. Laszak, C. Moulin, V. Moulin, C. Tondre, Appl. Spectrosc. to be published.

  15. S. Scapolan, E. Ansoborlo, C. Moulin, C. Madic, Intern. J. Radiat. Biol., to be published.

  16. S. Hjerten, Isoelectric Focusing in Capillaries, in: Capillary Electrophoresis: Theory and Practice,P. D. Grossman, J. C. Colburn (Ed.), Academic Press, San Diego, 1992.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut de Protection et de Sûreté Nucléaire, DPHD/SDOS, IPSN-BP no6, F-92265, Fontenay-aux-Roses Cedex, France

    S. Scapolan & E. Ansoborlo

  2. Laboratoire de Spectroscopie Laser Analytique, CEA/DCC/DPE/SPEA/SPS, CE/Saclay, 91191, Gif-sur-Yvette, France

    C. Moulin

  3. CEA/VALRHO-Marcoule, CEA/DCC/DRDD/SEMP, BP no 171, 30207, Bagnols sur Ceze Cedex, France

    C. Madic

Authors
  1. S. Scapolan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Ansoborlo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Moulin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. Madic
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scapolan, S., Ansoborlo, E., Moulin, C. et al. Uranium speciation in biological medium by means of capillary electrophoresis and time-resolved laser-induced fluorescence. J Radioanal Nucl Chem 226, 145–148 (1997). https://doi.org/10.1007/BF02063639

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02063639

Keywords

Search

Navigation