Abstract
Streptococcus lactis possessed glucose-6-phosphate-dehydrogenase, 6-phosphogluconic-dehydrogenase, ribose-5-phosphate isomerase and transketolase enzyme activities. The presence of the previously reported aldolase, lactic dehydrogenase, alcohol dehydrogenase and aldehyde dehydrogenase activities in the organism was confirmed. These results indicated that carbohydrate metabolism in the organism follows both Embden-Meyerhof-Parnas and Hexose monophosphate shunt pathways. The presence of aldolase, glucose-6-phosphate-dehydrogenase and 6-phosphogluconic-dehydrogenase is in accordance with the fact that the organism is a facultative homofermenter.
Antibiotics suppressed or retarded the activities of the above enzymes to different degrees and induced alterations in the glycolytic and alternate pathways of carbohydrate metabolism ofS.lactis.
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Abbreviations
- EMP:
-
Embden-Meyerhof-Parnas
- F-6-P:
-
fructose-6-phosphate
- Gal-1-P:
-
galactose-1-phosphate
- G-1-P:
-
glucose-1-phosphate
- G-6-P-D:
-
glucose-6-phosphate dehydrogenase
- Gly-3-P:
-
glyceraldehyde-3-phosphate
- HMS:
-
hexose monophosphate shunt
- NAD:
-
nicotinamide adenine dinucleotide
- NADH:
-
reduced nicotinamide adenine dinucleotide
- NADP:
-
nicotinamide adenine dinucleotide phosphate
- Sed-7-P:
-
sedoheptulose-7-phosphate
- 6-P-G-D:
-
6-phospho-gluconic dehydrogenase
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This work was supported in part by U.S. Public Health Service Research Grant E-2486, from the National Institute of Health. It is being published with the approval of the Director as paper No. 2528, Journal Series, Nebraska Agricultural Experiment Station, Lincoln.
Able technical assistance of Miss Clara Zoz is gratefully acknowledged.
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Vakil, J.R., Shahani, K.M. Carbohydrate metabolism of lactic acid cultures IV. Glycolytic and HMS pathway enzymes inStreptococcus lactis and their sensitivity to antibiotics. Antonie van Leeuwenhoek 36, 285–295 (1970). https://doi.org/10.1007/BF02069030
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DOI: https://doi.org/10.1007/BF02069030