Skip to main content
SpringerLink
Log in

Yeast protoplasts immobilized in alginate: Cell wall regeneration and reversion to cells

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Yeast protoplasts may regenerate the cell wall and revert to cells if immobilized in a 2%–5% Ca-alginate gel and cultured in an osmotically stabilized medium. The method of protoplast immobilization and subsequent isolation from the gel is described in detail. The reversion yield is dependent of the actual gel concentration, gel shape (beads vs. sheets) and of a medium molarity, and it may be up to 90%. The morphology of the cell wall regeneration and morphology of reversion to the cell forms correspond to protoplast development in gelatin or agar gels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Draget KI, Myhre S, Skjak-Braek G, Ostgaard K (1988) Regeneration, cultivation and differentiation of plant protoplasts immobilized in Ca-alginate beads. J Plant Physiol 132:552–556

    Google Scholar 

  2. Farkaš V (1988) Polysaccharide metabolism. In: Rose AH, Harrison JS (eds) The yeasts, vol 3, 2nd edn. London: Academic Press, pp 317–366

    Google Scholar 

  3. Johansen A, Flink JM (1986) Immobilization of yeast cells by internal gelation of alginate. Enzyme Micro Technol 8:145–148

    Google Scholar 

  4. Klein J, Wagner F (1983) Methods for the immobilization of microbial cells. In: Chibata I, Wingard LB (eds) Immobilization of microbial cells. Orlando: Academic Press, pp 11–51

    Google Scholar 

  5. Linsefors L, Brodelius P (1985) Immobilization of plant protoplasts: viability studies. Plant Cell Rep 4:23–27

    Google Scholar 

  6. Nečas O, Svoboda A (1985) Cell wall regeneration and protoplast reversion. In: Peberdy JF, Ferenczy L (eds) Fungal protoplasts. New York, Basel: Marcel Dekker Inc, pp 115–133

    Google Scholar 

  7. Smidsrod O (1972) Molecular bases for some physical properties of alginates in the gel state. Faraday Disc Soc 57:263–274

    Google Scholar 

  8. Svoboda A, Piedra D (1983) Reversion of yeast protoplasts in media containing polyethylene glycol. J Gen Microbiol 129:3371–3377

    Google Scholar 

  9. Tanaka H, Matsumura M, Veliky IA (1984) Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol Bioeng 26:53–58

    Google Scholar 

  10. Vidoli R, Yamazaki H, Nasim A, Veliky IA (1982) A novel procedure for the recovery of hybrid products from protoplast fusion. Biotechnol Lett 4:781–784

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Medical Faculty, J.E. Purkyne University, 66243, Brno, CSSR

    Augustin Svoboda & Petr Ouředníček

Authors
  1. Augustin Svoboda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Petr Ouředníček
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Svoboda, A., Ouředníček, P. Yeast protoplasts immobilized in alginate: Cell wall regeneration and reversion to cells. Current Microbiology 20, 335–338 (1990). https://doi.org/10.1007/BF02091915

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02091915

Keywords

Search

Navigation