Abstract
The performance of an ELISA for detection of total antibodies to Bruceila spp. was compared with that of the Rose Bengal, standard agglutination and Coombs test in the diagnosis of brucellosis. Sera tested were from 208 patients from whom Brucella melitensis had been isolated, 177 patients with significant results in at least two conventional tests, and 107 patients with fever from whom no Brucella spp. had been isolated and in whom all conventional tests were negative. ELISA was the most sensitive test (97 %), showing greater specificity (96 %) and good predictive positive and negative values (98 % and 94 % respecitvely). ELISA was the only positive test in 6 % of patients in whom brucellosis had been confirmed by culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buchanan, T. M., Sulzer, C. R., Frix, M. K., Feldman, R. A.: Brucellosis in the United States, 1960–1972. An abattoir-associated disease. II: Diagnostic aspects. Medicine 1974, 53: 415–425.
Marmonier, A., Sthal, J. P., Micoud, M., Le Noc, P.: Application de la technique immunoénzymatique ELISA au diagnostic serologique des brucelloses humaines. II: Comparaison aux autres techniques se'rologiques existantes. Pathologie et Biologie 1981, 29: 83–87.
Pellerin, J. L., Geral, M. F., Lautie, R.: Le test immunoénzymatique ELISA dans le diagnostique sérologique de la brucellose humaine. Etude comparative avec quatre techniques classiques. Revue de Médécine Véterinaire 1980, 131: 741–766.
De Klerk, E., Anderson, R.: Comparative evaluation of the enzyme-linked immunosorbent assay in the laboratory diagnosis of brucellosis. Journal of Clinical Microbiology 1985, 21: 381–386.
Gilbert, G. L., Hawes, L. A.: The antibody response toBruceila: immunoglobulin response measured by enzyme-linked immunosorbent assay and conventional tests. Australian and New Zealand Journal of Medicine 1981, 11: 40–45.
Chappel, R. J., Hayes, J.: Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis. Journal of Hygiene 1983, 90: 67–70.
Parrat, D., Nielsen, K. H., White, R. G., Payne, D. J. H.: Radioimmunoassay of IgM, IgG and IgABruceila antibodies. Lancet 1977, i: 1075–1078.
Lindberg, A. A., Haeggman, S., Karlson, K., Carlsson, H. E., Mair, N. S.: Enzyme immunoassay of the antibody response toBrucella andYersinia enterocolitica 0∶9 infection in humans. Journal of Hygiene 1982, 88: 295–307.
Magee, I. T.: An enzyme-labelled immunosorbent assay forBrucella abortus antibodies. Journal of Medical Microbiology 1980, 13: 167–172.
Bettelheim, K. A., Maskill, W. J., Pearce, J.: Comparison of standard tube and microagglutination techniques for determiningBrucella antibodies. Journal of Hygiene 1983, 90: 33–39.
Davies, G.: The Rose Bengal test. Veterinary Records 1971, 88: 447–449.
Otero, J. R., Fuertes, A., Palenque, E., Noriega, A. R.: Microtiter-adapted method that facilitates the Coombs test for brucellosis. Journal of Clinical Microbiology 1982, 16: 737–738.
Sippel, J. E., El-Masry, N. A., Farid, Z.: Diagnosis of human brucellosis with ELISA. Lancet 1982, ii: 19–21.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saz, J.V., Beltrán, M., Díaz, A. et al. Enzyme-linked immunosorbent assay for diagnosis of brucellosis. Eur. J, Clin. Microbiol. 6, 71–74 (1987). https://doi.org/10.1007/BF02097200
Issue Date:
DOI: https://doi.org/10.1007/BF02097200