Summary
The disulfide reducing activities of GSSG-and CoASSG-reductases were measured on partially purified extracts from a variety of prokaryotes and eukaryotes.
Glutathione-reductase was found in varying amounts in all eukaryotes and prokaryotes, used in this study, with the exception of the two strict anaerobesClostridium tartarivorum andDesulfovibrio vulgaris, and the two primitive ArchaebacteriaMethanosarcina barkeri andHalobacterium halobium.
CoASSG-reductase was found in some eukaryotes and prokaryotes, but showed no clear pattern of distribution other than its absence whenever GSSG-reductase was not present.
The absence of GSSG-reductase activity in organisms lacking GSH, confirms that glutathione metabolism is not universal and suggests that this enzyme might be useful as a marker in classifying organisms. The data suggest that glutathione-reductase occurs as a result of the change from a reducing to a oxidizing atmosphere in the primitive Earth.
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A great part of this work was carried out by R.N. Ondarza, during his sabbatical leave at Scripps Institution of Oceanography, La Jolla
Recipient of a grant by the Centro de Investigación en Química Aplicada at Saltillo, Coahuila, Mexico
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Ondarza, R.N., Rendón, J.L. & Ondarza, M. Glutathione reductase in evolution. J Mol Evol 19, 371–375 (1983). https://doi.org/10.1007/BF02101641
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DOI: https://doi.org/10.1007/BF02101641