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Effectors of the mammalian plasma membrane NADH-oxidoreductase system. Short-chain ubiquinone analogues as potent stimulators

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Abstract

In the presence of effectors variations in the two recognized activities of the plasma membrane NADH-oxidoreductase system were studied in separate, specificin vitro assays. We report here that ubiquinone analogues that contain a short, less hydrophobic side chain than coenzyme Q-10 dramatically stimulate the NADH-oxidase activity of isolated rat liver plasma membranes whereas they show no effect on the reductase activity of isolated membranes. If measured in assays of the NADH∶ferricyanide reductase of living cultured cells these compounds have only a limited effect; the oxidase activity of whole cells is not measurable in our hands. We have furthermore identified selective inhibitors of both enzyme activities. In particular, the NADH-oxidase activity can be significantly inhibited by structural analogues of ubiquinone, such as capsaicin and resiniferatoxin. The NADH∶ferricyanide reductase, on the other hand, is particularly sensitive to pCMBS, indicating the presence of a sulfhydryl group or groups at its active site. The identification of these specific effectors of the different enzyme activities of the PMOR yields further insights into the function of this system.

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Abbreviations

DCIP:

2,6-dichlorophenol-indophenol

DTNB:

5,5′-dithio-bis(2-nitrobenzoic acid)

FAC:

ferric ammonium citrate

pCMBS:

p-chloromercuriphenylsulfonic acid

PMOR:

plasma membrane NADH-oxidoreductase

coenzyme Q-0:

2,3-dimethoxy-5-methyl-1,4-benzoquinone

SH:

sulfhydryl group

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Author notes

  1. Gabrielle L. McMullen

    Present address: Australian Catholic University, Aquinas Campus, 3350, Ballarat, VIC, Australia

  2. Ernst J. Wolvetang

    Present address: Monash Medical Centre, Institute for Reproduction and Development, 266 Clayton Road, 3168, Clayton, VIC, Australia

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, 3168, Clayton, Victoria, Australia

    FranÇois Vaillant, Jari A. Larm, Gabrielle L. McMullen, Ernst J. Wolvetang & Alfons Lawen

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  1. FranÇois Vaillant
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  2. Jari A. Larm
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  3. Gabrielle L. McMullen
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  4. Ernst J. Wolvetang
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  5. Alfons Lawen
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Vaillant, F., Larm, J.A., McMullen, G.L. et al. Effectors of the mammalian plasma membrane NADH-oxidoreductase system. Short-chain ubiquinone analogues as potent stimulators. J Bioenerg Biomembr 28, 531–540 (1996). https://doi.org/10.1007/BF02110443

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