Abstract
Cultured chick embryonal retinal pigment epithelial (RPE) cells synthesized two main glycosaminoglycans (GAGs), chondroitin 4-sulfate, and heparan sulfate. Chondroitin 4-sulfate was liberated mainly in the medium (apical side) and heparan sulfate was incorporated into the cell and/or basement membrane. Glycosaminoglycans liberated into the medium were composed of chondroitin 4-sulfate (44%), keratan sulfate (22%), heparan sulfate (18%) and hyaluronate (12%). On the other hand, the GAGs in the cell and/or basement membrane were composed of heparan sulfate (52%), hyaluronate (19%), and chondroitin 4-sulfate (13%).
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Chihara, E., Tsukada-Fujioka, T. & Tsukahara, I. Synthesis of glycosaminoglycans by chick retinal pigment epithelium in vitro. Graefe's Arch Clin Exp Ophthalmol 222, 45–50 (1984). https://doi.org/10.1007/BF02133778
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DOI: https://doi.org/10.1007/BF02133778