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The determination of orotic acid in the blood serum by means of the spectrophotometric method

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  • Chimica. Biochimica. Biophysica
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Zusammenfassung

Das Prinzip des Orotsäurenachweises beruht auf der Deproteination des Serums mittels Uranylessigester und der Verwandlung der Orotsäure durch Bromation in 5,5-Dibrombarbitursäure und nachfolgende Debromation in Barbitursäure. Letztere ergibt mitp-Dimethylaminobenzaldehyd ein intensiv gefärbtes Produkt, 5-(p-dimethylaminobenzyliden)-Barbitursäure.

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Stajner, A., Sůva, J. & Musil, F. The determination of orotic acid in the blood serum by means of the spectrophotometric method. Experientia 24, 116–117 (1968). https://doi.org/10.1007/BF02146929

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