Abstract
Pistils ofNicotiana alata (Link et Otto) contain an abundant, style-specific glycoprotein (120 kDa) that is rich in hydroxyproline and has both extensin-like and arabinogalactan-protein-like carbohydrate substituents. An antibody specific for the protein backbone of the glycoprotein was used to localise the glycoprotein in both unpollinated and pollinated pistils. The glycoprotein is evenly distributed in the extracellular matrix of the style transmitting tract of unpollinated pistils and, despite the presence of extensin-like carbohydrate substituents, is not associated with the walls of the transmitting tract cells. In pollinated pistils the 120-kDa glycoprotein is concentrated in the extracellular matrix adjacent to pollen tubes, and is also present in the cytoplasm and the cell walls of pollen tubes. Pollen tubes grown in vitro do not contain the 120-kDa glycoprotein unless it is added to the growth medium, suggesting that the 120kDa glycoprotein located in pistil-grown pollen tubes is derived from the extracellular matrix of the transmitting tract.
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References
Anderson MA, Harris PJ, Bönig I, Clarke AE (1987) Immunogold localization of α-L-arabinofuranosyl residues in pollen tubes ofNicotiana alata Link et Otto. Planta 171:438–442
Anderson MA, McFadden GI, Bernatzky R, Atkinson A, Orpin T, Dedman H, Tregear G, Fernley R, Clarke AE (1989) Sequence variability of three alleles of the self-incompatibility gene ofNicotiana alata. Plant Cell 1:483–491
Aspinall GO, Rosell KG (1978) Polysaccharide component in the stigmatic exudate fromLilium longiflorum. Phytochemistry 17:919–921
Atkinson AH, Lind JL, Clarke AE, Anderson MA (1994) Molecular and structural features of the pistil ofNicotiana alata. Biochem Soc Symp 60:15–26
Capkova V, Hrabetova E, Tupy J, Rihova L (1983) Amino acid uptake and protein synthesis in cultured tobacco pollen. Biochem Physiol Pflan 178:511–520
Chen C-G, Cornish EC, Clarke AE (1992) Specific expression of an extensin-like gene in the style ofNicotiana alata. Plant Cell 4:1053–1062
Chen C-G, Mau S-L, Clarke AE (1993) Nucleotide sequence and style-specific expression of a novel proline-rich gene fromNicotiana alata. Plant Mol Biol 21:391–395
Chen M-S, Loewus FA (1977)myo-Inositol metabolism inLilium longiflorum pollen. Uptake and incorporation of myo-inositol2-3H. Plant Physiol 59:653–657
Cresti M, Keijzer CJ, Tiezzi A, Ciampolini F, Focardi S (1986) Stigma ofNicotiana: ultrastructural and biochemical studies. Am J Bot 73:1713–1722
Cresti M, Went JL van (1976) Callose deposition and plug formation inPetunia pollen tubes in situ. Planta 133:35–40
Deshusses J, Gumber SC, Loewus FA (1981) Sugar uptake in lily pollen. Plant Physiol 67:793–796
Gleeson PA, Clarke AE (1979) Structural studies on the major component ofGladiolus style mucilage, an arabinogalactan-protein. Biochem J 181:607–621
Goldman MH de S, Pezzotti M, Seurinck J, Mariani C (1992) Developmental expression of tobacco pistil-specific genes encoding novel extensin-like proteins. Plant Cell 4:1041–1051
Gray JE, McClure BA, Bönig I, Anderson MA, Clarke AE (1991) Action of the style product of the self-incompatibility gene ofNicotiana alata (S-RNase) on in vitro-grown pollen tubes. Plant Cell 3:271–283
Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
He D, Simpson RJ, Moritz RL, Clarke AE, Bacic A (1994) Isolation of the protein backbone of an arabinogalactan-protein from the styles ofNicotiana alata and characterisation of a corresponding cDNA. Plant Cell 6:1643–1653
Hoggart RM, Clarke AE (1984) Porosity ofGladiolus stigmatic papillae and pollen tube walls. Ann Bot 53:271–277
Huang S, Lee H-S, Karunanandaa B, Kao T-H (1994) Ribonuclease activity ofPetunia inflata S proteins is essential for rejection of self-pollen. Plant Cell 6:1021–1028
Knox JP (1990) Emerging patterns of organisation at the plant cell surface. J Cell Sci 96:557–561
Konar RN, Liskens HF (1966) Physiology and biochemistry of the stigmatic fluid ofPetunia hybrida. Planta 71:372–387
Kowyama Y, Kunz C, Lewis I, Newbigin E, Clarke AE, Anderson MA (1994) Self-compatibility in aLycopersicon peruvianum variant (LA2157) is associated with lack of style S-RNase activity. Theor Appl Genet 88:859–864
Labarca C, Kroh M, Loewus F (1970) The composition of stigmatic exudate fromLilium longiflorum. Labelling studies with myo-inositol, D-glucose and L-proline. Plant Physiol 46:150–156
Labarca C, Loewus F (1972) The nutritional role of pistil exudate in pollen tube wall formation inLilium longiflorum. I. Utilization of injected stigmatic exudate. Plant Physiol 50:7–14
Labarca C, Loewus F (1973) The nutritional role of pistil exudate in pollen tube wall formation inLilium longiflorum. II. Production and utilization of exudate from the stigma and stylar canal. Plant Physiol 52:87–92
Li YA, Chen F, Linskens HF, Cresti M (1994) Distribution of unesterified and esterified pectins in cell walls of pollen tubes of flowering plants. Sex Plant Reprod 7:145–152
Lind JL, Bacic A, Clarke AE, Anderson MA (1994) A style-specific hydroxyproline-rich glycoprotein with properties of both extensins and arabinogalactan proteins. Plant J 6:491–502
Loewus F, Labarca C (1973) Pistil secretion product and pollen tube wall formation. In: Loewus F (ed) Biogenesis of plant cell wall polysaccharides. Academic Press, New York, pp 175–193
Maheswaran G, Perryman T, Williams EG (1986) Use of an interspecific hybrid in identifying a new allelic specificity generated at the self-incompatibility locus after inbreeding inLycopersicon peruvianum. Theor Appl Genet 73:236–245
McClure BA, Gray JE, Anderson MA, Clarke AE (1990) Self-incompatibility inNicotiana alata involves degradation of pollen rRNA. Nature 347:757–760
Nettancourt D de, Devreux M, Bozzini A, Cresti M, Pacini E, Sarfatti G (1973) Ultrastructural aspects of the self-incompatibility mechanism inLycopersicon peruvianum Millennium. J Cell Sci 12:430–419
Newbigin E, Anderson MA, Clarke AE (1993) Gametophytic self-incompatibility systems. Plant Cell 5:1315–1324
O'Driscoll D, Hann C, Read SM, Steer MW (1993a) Endocytotic uptake of fluorescent dextrans by pollen tubes grown in vitro. Protoplasma 175:126–130
O'Driscoll D, Read SM, Steer MW (1993b) Determination of cellwall porosity by microscopy: walls of cultured cells and pollen tubes. Acta Bot Neerl 42:237–244
Read SM, Bacic A (1995) Cell wall porosity and its determination. In: Jackson JF, Linskens H-F (eds) Plant cell wall analysis. (Modern methods of plant analysis, vol 17). Springer, Berlin Heidelberg New York (in press)
Read SM, Bacic A, Clarke AE (1992) Pollen tube growth in culture. I. Control of morphology and generative nucleus division in cultured pollen tubes ofNicotiana. In: Ottaviano E, Mulcahy DL, Sari Gorla M, Mulcahy GB (eds) Angiosperm pollen and ovules. Springer, Berlin Heidelberg New York, pp 162–167
Royo J, Kunz C, Kowyama Y, Anderson M, Clarke AE, Newbigin E (1994) Loss of a histidine residue at the active site of S-locus ribonuclease is associated with self-compatibility inLycopersicon peruvianum. Proc Natl Acad Sci USA 91:6511–6514
Schlüpmann H, Bacic A, Read SM (1994) Uridine diphosphate glucose metabolism and callose synthesis in cultured pollen tubes ofNicotiana alata Link et Otto. Plant Physiol 105:659–670
Smith JJ, Muldoon EP, Lamport DTA (1984) Isolation of extensin precursors by direct elution of intact tomato cell suspension cultures. Phytochemistry 23:1233–1239
Stuart DA, Varner JE (1980) Purification and characterization of a salt-extractable hydroxyproline-rich glycoprotein from aerated carrot discs. Plant Physiol 66:787–792
Tupy J (1961) Investigation of free amino-acids in cross-, self-, and non-pollinated pistils ofNicotiana alata. Biol Plantarum 3:47–64
Wang H, Wu H-M, Cheung AY (1993) Developmental and pollination regulated accumulation and glycosylation of a stylar transmitting tissue-specific proline-rich protein. Plant Cell 5:1639–1650
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Lind, J.L., Bönig, I., Clarke, A.E. et al. A style-specific 120-kDa glycoprotein enters pollen tubes ofNicotiana alata in vivo. Sexual Plant Reprod 9, 75–86 (1996). https://doi.org/10.1007/BF02153054
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DOI: https://doi.org/10.1007/BF02153054