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Linear models relating xanthophylls and lumen acidity to non-photochemical fluorescence quenching. Evidence that antheraxanthin explains zeaxanthin-independent quenching

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Abstract

Zeaxanthin has been correlated with high-energy non-photochemical fluorescence quenching but whether antheraxanthin, the intermediate in the pathway from violaxanthin to zeaxanthin, also relates to quenching is unknown. The relationships of zeaxanthin, antheraxanthin and ΔpH to fluorescence quenching were examined in chloroplasts ofPisum sativum L. cv. Oregon andLactuca sativa L. cv. Romaine. Data matrices as five levels of violaxanthin de-epoxidation against five levels of light-induced lumen-proton concentrations were obtained for both species. The matrices included high levels of antheraxanthin as well as lumen-proton concentrations induced by subsaturating to saturation light levels. Analyses of the matrices by simple linear and multiple regression showed that quenching is predicted by models where the major independent variable is the product of lumen acidity and de-epoxidized xanthophylls, the latter as the sum of zeaxanthin and antheraxanthin. The interactions of lumen acidity and xanthophyll concentration are shown in three-dimensional plots of the best-fit multiple regression models. Antheraxanthin apparently contributes to quenching as effectively as zeaxanthin and explains quenching previously not accounted for by zeaxanthin. Hence, we propose that all high-energy dependent quenching is xanthophyll dependent. Quenching requires a threshold lumen pH that varies with xanthophyll composition. After the threshold, quenching is linear with lumen acidity or xanthophyll composition.

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Abbreviations

FM :

fluorescence intensity with PS II traps closed under non-energized, dark-adapted conditions

F′M :

fluorescence intensity with PS II traps closed in energized state

F0 :

fluorescence intensity with PS II reaction centers open in non-energized conditions

NPQ:

non-photochemical quenching

PFD:

photon flux density

SVN :

Stern Volmer non-photochemical quenching

SVE :

Stern Volmer energy-dependent non-photochemical quenching

qE :

coefficient of energy-dependent quenching

kD :

rate constant for non-radiative energy dissipation

V:

violaxanthin

A:

antheraxanthin

Z:

zeaxanthin

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  1. Department of Plant Molecular Physiology, University of Hawaii at Manoa, 3190 Maile Way, St. John 503, 96822, Honolulu, Hawaii, USA

    Adam M. Gilmore & Harry Y. Yamamoto

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  1. Adam M. Gilmore
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  2. Harry Y. Yamamoto
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Gilmore, A.M., Yamamoto, H.Y. Linear models relating xanthophylls and lumen acidity to non-photochemical fluorescence quenching. Evidence that antheraxanthin explains zeaxanthin-independent quenching. Photosynth Res 35, 67–78 (1993). https://doi.org/10.1007/BF02185412

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  • DOI: https://doi.org/10.1007/BF02185412

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