Skip to main content
SpringerLink
Log in

Relationship between substrate inhibition and maintenance energy ofChlamydomonas reinhardtii in heterotrophic culture

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

The relationship between substrate inhibition and maintenance energy ofChlamydomonas reinhardtii grown heterotrophically on acetate was investigated. At low acetate concentrations (<0.4 g l−1), where no inhibition of cell growth was observed, the cell growth yield and specific growth rate could be represented by the Pirt model, 1/Y=1/Y g +m/μ with a constant value of maintenance energy coefficient m. However, at high acetate concentrations (>0.4 g l−1), inhibition of cell growth occurred, in which m became variable and dependent on the acetate concentration. A simple mathematical model was proposed to predict the actual maintenance energy coefficient m in the inhibited cultures and experimentally validated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Borowitzka MA (1994) Products from algae. In Phang SM, Lee YK, Borowitzka MA, Whitton BA (eds), Algal Biotechnology in the Asia-Pacific Region. University of Malaya, Kuala Lumpur: 5–15.

    Google Scholar 

  • Chen F (1993) High Cell Density Culture ofChlamydomonas reinhardtii on Acetate under Heterotrophic Conditions of Growth. Ph.D. Thesis, The University of Queensland.

  • Chen F, Johns MR (1994a) Substrate inhibition ofChlamydomonas reinhardtii by acetate in heterotrophic culture. Process Biochem. 29: 245–252.

    Google Scholar 

  • Chen F, Johns MR (1994b) Substrate inhibition kinetics of heterotrophicChlamydomonas reinhardtii. In Phang SM, Lee YK, Borowitzka MA, Whitton BA (eds), Algal Biotechnology in the Asia-Pacific Region. University of Malaya, Kuala Lumpur: 134–139.

    Google Scholar 

  • Chen F, Johns MR (1995) A strategy for high cell density culture of heterotrophic microalgae with inhibitory substrates. J. appl. Phycol. 7: 43–46.

    Google Scholar 

  • Cutayar JM, Poillon D (1989) High cell density culture ofE. coli in a fed-batch system with dissolved oxygen as substrate feed indicator. Biotechnol. Lett. 11: 155–160.

    Google Scholar 

  • Fieschko J, Humphrey AE (1983) Effects of temperature and ethanol concentration on the maintenance and yield coefficient ofZymomonas mobilis. Biotechnol. Bioengng 25: 1655–1660.

    Google Scholar 

  • Harrison DEF, Topiwapa HH (1974) Transient and oscillatory states of continuous culture. Adv. Biochem. Eng. 3: 167–219.

    Google Scholar 

  • Johns MR, Tan CK, Chen F (1989) Heterotrophic microalgal production of chemicals. Proc. Eighth Australian Biotechnology Conference. Univ. of New South Wales, Sydney: 542–545.

    Google Scholar 

  • Lee KJ, Skotnicki ML, Tribe DE, Rogers PL (1980) Kinetic studies on a highly productive strain ofZymomonas mobilis. Biotechnol. Lett. 2: 339–344.

    Google Scholar 

  • Maiorella B, Blanch HW Wilke CR (1983) By-product inhibition effects on ethanolic fermentation bySaccharomyces cerevisiae. Biotechnol. Bioengng 25: 103–121.

    Google Scholar 

  • Pirt SJ (1965) The maintenance energy of bacteria in growing cultures. Proc. Roy. Soc. Lond. B163: 224–231.

    Google Scholar 

  • Pirt SJ (1975) Principles of Microbe and Cell Cultivation. Halsted Press, New York.

    Google Scholar 

  • Pirt SJ (1982) Maintenance energy: a general model for energy-limited and energy-sufficient growth. Arch Microbiol. 133: 300–302.

    Google Scholar 

  • Richmond A (1992) Open systems for the mass production of photoautotrophic microalgae outdoors: physical principles. J. appl. Phycol. 4: 281–286.

    Google Scholar 

  • Sager R, Granick S (1953) Nutritional studies withChlamydomonas reinhardtii. Annals New York Academy of Sciences 56: 831–838.

    Google Scholar 

  • Starr RC, Zeikus JA (1993) UTEX-the culture collection of algae at the University of Texax at Austin. J. Phycol. 29 (suppl.): 1–106.

    Google Scholar 

  • Stouthamer AH (1979) The search for correlation between theoretical and experimental growth yields. International Review Biochemistry 21: 1–48.

    Google Scholar 

  • Tseng MM, Wayman M (1975) Kinetics of yeast growth: inhibition-threshold substrate concentrations. Can. J. Microbiol. 21: 994–1003.

    Google Scholar 

  • Yang ST, Okos MR (1987) Kinetic study and mathematical modeling of methanogenesis of acetate using pure culture of methanogens. Biotechnol. Bioengng 30: 661–667.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong

    Feng Chen

  2. Department of Chemical Engineering, The University of Queensland, Brisbane, QLD 4072, Australia

    Michael R. Johns

Authors
  1. Feng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael R. Johns
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Author for correspondence

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, F., Johns, M.R. Relationship between substrate inhibition and maintenance energy ofChlamydomonas reinhardtii in heterotrophic culture. J Appl Phycol 8, 15–19 (1996). https://doi.org/10.1007/BF02186216

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02186216

Key words

Search

Navigation