Skip to main content
Log in

Evaluation of extractants for Zn and Cu in paddy soils

  • Published:
Plant and Soil Aims and scope Submit manuscript

Summary

A pot experiment was conducted with two rice varieties grown under flooded conditions to evaluate five chemical extractants,viz: 1N NH4OAc (pH 4.6), 0.01M EDTA-1M (NH4)2CO3 (pH 8.6), 0.05N HCl, 0.01M CaCl2 and 0.2M MgSO4 for extraction of ‘available’ Zn and Cu. Soil Zn and Cu extracted (a) from dry soils before puddling and (b) from wet soils at four and eight week after transplanting were correlated with plant Cu and Zn contents. All extractants gave significant correlations with plant Zn. Highest correlation was obtained with NH4OAc followed by HCl. Zinc extracted from dry soils correlated significantly with that from wet soils. Extractable Zn decreased slightly from zero to four weeks after flooding but increased subsequently. Ammonium acetate, HCl and EDTA extracted approximately equal amounts of Zn.

The EDTA was most suitable for Cu followed by NH4OAc and HCl. Although EDTA extracted more Cu than the other extractants, the generally low ‘r’ values obtained suggested a need for further experimentation.

Zinc concentration in plants did not vary significantly with age but Cu concentration declined from fourth to eighth week after transplanting.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Black C A (Ed.) 1965 Methods of Soil Analysis. Part 2. Am. Soc. Agron. Inc. Madison, USA.

    Google Scholar 

  2. Dhingra D R and Kanwar J S 1963 Status of Zn in Punjab soils. Punjab Hortic. J. 3, 54–59.

    Google Scholar 

  3. Gangwar M S and Chandra S K 1976 Estimation of critical limit for zinc in rice soils. Commun. Soil Sci. Plant Anal. 7, 225–310.

    Google Scholar 

  4. Giordano P M and Mortvedt J J 1974 Response of several rice cultivars to Zn. Agron. J. 66, 220–223.

    Google Scholar 

  5. Grewal J S, Randhawa N S and Bhumbla D R 1967 Correlation of soil test with wheat response to the application of zinc. J. Ind. Soc. Soil Sci. 15, 202.

    Google Scholar 

  6. Haq A U, Bates T E and Soon Y K 1980 Comparison of extractants for plant available zinc, cadmium, nickel and copper in contaminated soils. Soil Sci. Soc. Am. J. 44, 772–777.

    Google Scholar 

  7. Hoeft R G, Walsh L M and Keeney D R 1973 Evaluation of various extractants for available soil sulphur. Soil Sci. Soc. Am. Proc. 37, 401–404.

    Google Scholar 

  8. International Rice Research Institute. 1971 Annual Report for 1970. Los Banos, Philippines. pp 31–40.

  9. Kang B T and Okoro E G 1976 Response of flooded rice grown on a vertisol from Northern Nigeria to zinc sources and methods of application. Plant and Soil 44, 15–25.

    Google Scholar 

  10. Katyal J C and Ponnamperuma F N 1974 Zinc deficiency. A widespread nutritional disorder of rice in Agusan del Norte. J. Philipp. Agric. 58, 79–89.

    Google Scholar 

  11. Lindsay W L and Norvell W A 1978 Development of a DTPA test for zinc, iron, manganese and copper. Soil Sci. Soc. Am. J. 42, 421–428.

    Google Scholar 

  12. Martens D C, Chesters G and Peterson L A 1966 Factors controlling the extractability of soil Zn. Soil Sci. Soc. Am. Proc. 30, 67–69.

    Google Scholar 

  13. Maskina M S, Randhawa N S and Sinha M K 1980 Relation of growth and zinc uptake of rice to quantity, intessity and buffering capacity factors of zinc in soils. Plant and Soil 54, 195–205.

    Google Scholar 

  14. Matt K John 1960 Extractable and plant available Zn in horizons of several Fraser river alluvial soils. Can. J. Soil Sci. 54, 125–132.

    Google Scholar 

  15. Misra S G and Misbra M K 1967 Availability of copper in some soils of Uttar Pradesh. J. Ind. Soil Sci. Soc. 17, 283–284.

    Google Scholar 

  16. Pavanasasivam V and Kalpage F S C P 1974 Status of Zn and Cu in the wet-patana soils of Sri Lanka. Trop. Agric. CXXX, 384.

    Google Scholar 

  17. Pavanasasivam V and Axley J H 1980 Influence of flooding on the availability of soil Zn. Commun. Soil Sci. Plant Anat. 11, 163–174.

    Google Scholar 

  18. Pinta M 1978 Modern methods for trace element analysis. Ann Arbor Sci. Publ., Inc. Ann Arbor, Michigan pp 207–263.

    Google Scholar 

  19. Ponnamperuma F N 1977 The behavior of minor elements in paddy soils. IRRI Research Paper Series No. 8, 15 p.

  20. Ranadive S J, Naik M S and Das N B 1964 Copper and zinc status of Maharashtra soils. J. Ind. Soil Sci. Soc. 12, 243–248.

    Google Scholar 

  21. Ruby Uy Castro 1976 Zinc deficiency in rice: A review of research at the International Rice Research Institute. IRRI Research Paper Series No. 9.

  22. Stewart J A and Berger K C 1970 Estimation of available soil Zn using MgCl2 as extractant. Soil Sci. 100, 244–250.

    Google Scholar 

  23. Tanga A and Yoshida S 1970 Nutritional disorders of rice plants in Asia. Tech. Bull. 10 IRRI, Philippines.

  24. Tobia S K and Hanna A S 1958 Effect of copper sulphate added to irrigation water on copper status of Egyptian soils. I. Amount of copper retained by soils. Soil. Sci. 85, 302–306.

    Google Scholar 

  25. Trierweiler J F and Lindsay W L 1969 EDTA-ammonium carbonate soil test for zinc. Soil Sci. Soc. Am. Proc. 33, 49–54.

    Google Scholar 

  26. Viets Jr F G 1962 Chemistry and availability of micronutrients in soils. J. Agric. Food Chem. 10, 174–178.

    Google Scholar 

  27. Viro P J 1955 Use of ethylenediaminetetraacetic acid in soil analysis. II. Determination of soil fertility. Soil Sci. 80, 69–74.

    Google Scholar 

  28. Wear J I and Evans C E 1968 Relationship of Zn uptake by corn and sorghum to soil Zn measured by three extractants. Soil Sci. Soc. Am. Proc. 32, 543–546.

    Google Scholar 

  29. Westfall D G, Flinchum W T and Stansel J W 1973 Distribution of nutrients in the rice plant and effect of two nitrogen levels. Agron. J. 65, 234–235.

    Google Scholar 

  30. Yoon S K, Gilmour J T and Wells B R 1975 Micronutrient levels in the rice plant leaf as a function of soil solution concentration. Soil Sci. Soc. Am. Proc. 39, 685–688.

    Google Scholar 

  31. Yoshida S, Forno D A and Bhadrachalam A 1971 Zinc deficiency of the rice plant on calcareous and neutral soils in the Philippines. Soil Sci. Plant Nutr. Japan 17, 83–87.

    Google Scholar 

  32. Yoshida S, Ahn J S and Forno D A 1973 Occurrence, diagnosis and correction of zinc deficiency of lowland rice. Soil Sci. Plant Nutr. Japan 19, 83–93.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Selvarajah, N., Pavanasasivam, V. & Nandasena, K.A. Evaluation of extractants for Zn and Cu in paddy soils. Plant Soil 68, 309–320 (1982). https://doi.org/10.1007/BF02197936

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02197936

Key words

Navigation