Summary
Ossein-hydroxyapatite compound (OHC) is a protein-mineral complex derived from bovine bone. Its effects on bone remodeling were studied in old ewes which have seasonal variations in bone remodeling. Seven animals received 200 mg OHC/kg b.w./day for 90 days from July to September. The control group consisted of 7 untreated animals followed for the same period of time. OHC was administered through a fistula into the fourth stomach. A significant decrease of bone histomorphometric parameter values was noted in controls at the end of the experiment, due to seasonal variations: the cancellous eroded perimeter decreased by 45%, the osteoblastic perimeter by 60% and the bone formation rate at the cell level by 20%. In contrast, in the treated-group, these parameters tended to increase or did not change. In conclusion, counteracting the significant seasonal reduction of bone remodeling in ewes, OHC seems able to stimulate directly or indirectly bone metabolism, especially when osteoblast activity is reduced and may partly prevent the seasonal reduction of bone turnover.
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References
Stephan, J.J., Pospichal, J., Presl, J., Pacousky, V. Prospective trial of ossein hydroxyapatite compound in surgically-induced postmenopausal women. Bone 1989, 10, 179–185.
Gunther, H., Fleisch, H. The effect of a bone matrix factor, isolated and partially purified from OSSOPAN® on the proliferation of isolated bone cells in vivo. 1984, Study report of the Institute for Pathophysiology of the University, Bern, Switzerland.
Annefeld, M., Caviezel, R., Schacht, E., Schicketanz, K.H. The influence of ossein-hydroxyapatite compound (“OSSOPA”) on the healing of a bone defect. Curr Med Res Opin 1986, 10, 241–250.
Schmidt, K.H., Wörner, U.M., Buck, H.J. Examination of new bone growth on aluminium oxide implant contact surfaces after oral administration of ossein-hydroxyapatite compound to rats. Curr Med Res Opin 1987, 11, 107–115.
Pastoureau, P., Merle, B., Delmas, P.D. Specific radioimmunoassay for ovine bone-Gla-protein. Acta Endocrinol (Copenh.) 1988, 113, 152–160.
Diehl, M., Ellingboe, J. Indicator for titration of calcium in presence of magnesium using disodium dihydrogen ethylene diamine tetracetate. Clin Chem 1950, 28, 882–884.
Fiske, C.H., Subbarow, Y. The colorimetric determination of phosphorus. J Biol Chem 1925, 66, 375–380.
Conaway, J.J., Anast, C.S. Double antibody radioimmunoassay for parathyroid hormone. J Lab Clin Med 1974, 83, 129–140.
Meunier, P.J. Histomorphometry of the skeleton. In: Bone and Mineral Research, Annual 1, Ed: Peck W.A., Amsterdam, Excerpta Medica, 1983, 191–222.
Schenk, R.K., Olah, A.J., Hermann, W. Preparation of calcified tissues for light microscopy. In: Methods of Calcified Tissue Preparation, Ed: Dickson, G.R., Amsterdam, Elsevier, 1984, 1–56.
Chavassieux, P.M., Arlot, M.E., Meunier, P.J. Intermethod variation in bone histomorphometry: comparison between manual and computerized method applied to iliac bone biopsies. Bone 1985, 6, 221–229.
Parfitt, A.M., Drezner, M.K., Glorieux, F.H., Kanis, J.A., Malluche, H., Meunier, P.J., Ott, S.M., Recker, R.R. Bone histomorphometry standardization of nomenclature, symbols and units. J Bone Min Res 1987, 2, 565–610.
Kragstrup, J., Gundersen, H.J.G., Melsen, F., Mosekilde, L. Estimation of the three-dimensional wall thickness of completed remodeling sites in iliac trabecular bone. Metab Bone Dis Rel Res 1982, 4, 113–119.
Vedi, S., Compston, J.E., Webb, A., Tighe, J.R. Histomorphometric analysis of dynamic parameters of trabecular bone formation in the iliac crest of normal British subjects. Metab Bone Dis Rel Res 1983, 5, 69–74.
Melsen, F., Mosekilde, L. Trabecular bone mineralization lag time determined by tetracycline double-labeling in normal and certain pathological conditions. Acta Pathol Microbiol Scand 1980, A88, 83–88.
Chavassieux, P., Pastoureau, P., Boivin, G., Charhon, S., Chapuy, M.C., Delmas, P.D., Meunier, P.J. Effects of sodium fluoride on bone remodeling in ewes. J Bone Min Res 1987, 2, Suppl. 1, 359.
Pastoureau, P., Arlot, M.E., Caulin, F., Barlet, J.P., Meunier, P.J., Delmas, P.D. Effects of oophorectomy on biochemical and histological indices of bone turnover in ewes. J Bone Min Res 1989, 4 (Suppl 1), 477.
Thimonier, J., Mauleon, P. Variations saisonnières du comportement d'oestrus et des activités ovarienne et hypophysaire chez les ovins. Ann Biol Anim Bioch Bioph 1969, 9, 233–250.
Annefeld, M. Les effets du “Protein-Mineral-Complex” (TWY-DIL R PMC) sur les structures et les fonctions des os et des cartilages. In: High Performance Horses Scientific News, Ed: Pavesco, A.G.. Liestal, Grawviller Offsetdruck, 1988, 16–24.
Fleisch, H. Study report on the effects of bone matrix derived polypeptides, isolated from OSSOPAN® on proliferation, collagen and glycosaminoglycan synthesis of isolated bone cells in vitro. Study Report of the Institute for Pathophysiology of the University, Bern, Switzerland, 1986.
Lietzke, R. Rapid determination of transforming growth factor type beta in monolayer culture. Anticancer Research 1987, 7, 5a.
Lietzke, R. Wachstumfaktoren im OSSOPAN-Nachweis und identifizeirung von TGF-B (Transforming growth factor type β) durch einen neuentwickelten bioassay. Study Report, ROBAPHARM LTD, 1987.
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Chavassieux, P., Pastoureau, P., Boivin, G. et al. Effects of ossein-hydroxyapatite compound on ewe bone remodeling: Biochemical and histomorphometric study. Clin Rheumatol 10, 269–273 (1991). https://doi.org/10.1007/BF02208688
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DOI: https://doi.org/10.1007/BF02208688