Abstract
Pseudomonas aeruginosa (strain 473) constitutively contains a soluble NADP-linked dehydrogenase active towards primary alcohols. In addition, at least two NAD(P)-independent primary alcohol dehydrogenases can be induced by growing this strain on primary alcohols,α,ω-diols orn-alkanes. These inducible enzymes were found to be bound to cellular structures. They reduce bovine cytochromec and various dyes, but not oxygen. The main difference between the inducible enzymes is their different capacity to oxidize ethanol. Noteworthy properties of the enzymes are:
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1)
the affinities for the straight-chain primary alcohols increase with increasing chain length (tested up to 1-decanol);
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2)
the affinities decrease when polar atoms or groups are introduced into the alcohol molecule;
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3)
enzyme preparations as well as intact cells, when provided with a mixture of alcohols, first oxidize the compound with the lowest solubility in water.
These properties can be explained by assuming that hydrophobic bonds are formed between the enzyme and aliphatic parts of the alcohol molecule.
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References
Anthony, C. andZatman, L. J. 1967. The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase ofPseudomonas sp. M d27: a new oxidoreductase prosthetic group. - Biochem. J.104: 960–969.
Azoulay, E. andHeydeman, M. T. 1963. Extraction and properties of alcohol dehydrogenase fromPseudomonas aeruginosa. - Biochim. Biophys. Acta73: 1–6.
Baptist, J. N., Gholson, R. K. andCoon, M. J. 1963. Hydrocarbon oxidation by a bacterial enzyme system. I. Products of octane oxidation. - Biochim. Biophys. Acta69: 40–47.
Dixon, M. andWebb, E. C. 1964. Enzymes. 2nd ed. p. 75. - Longmans, Green and Co. Ltd. London and Colchester.
Heydeman, M. T. andAzoulay, E. 1963. Extraction and properties of aldehyde dehydrogenase fromPseudomonas aeruginosa. - Biochim. Biophys. Acta77: 545–553.
Janota-Bassalik, L. andWright, L. D. 1964. Azelaic acid utilization by aPseudomonas. - J. Gen. Microbiol.36: 405–414.
van der Linden, A. C. 1967. Dissimilation of 1,6-hexanediol and 1,8-octanediol by a hydrocarbon-oxidizingPseudomonas.- Antonie van Leeuwenhoek33: 386–396.
Lowry, O. H., Rosebrough, N. J., Farr, L. A. andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. - J. Biol. Chem.193: 265–275.
Payne, W. J. 1963. Pure culture studies of the degradation of detergent compounds. - Biotechnol. Bioeng.5: 355–365.
Sund, H. andTheorell, H. 1963. Alcohol dehydrogenases, p. 25–83.In P. D. Boyer, H. Lardy and K. Myrbäck, [eds.]. The Enzymes, 2nd. ed., Vol. 7. - Academic Press, New York and London.
Thysse, G. J. E. andvan der Linden, A. C. 1958.n-Alkane oxidation by aPseudomonas. Studies on the intermediate metabolism. - Antonie van Leeuwenhoek24: 298–308.
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van der Linden, A.C., Huybregtse, R. Occurrence of inducible and NAD(P)-independent primary alcohol dehydrogenases in an alkane-oxidizingPseudomonas . Antonie van Leeuwenhoek 35, 344–360 (1969). https://doi.org/10.1007/BF02219154
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DOI: https://doi.org/10.1007/BF02219154