Summary
Based on the previously observed proportionality between the retention constant of some mono-and polyfunctional steroids and the retention constant of the steroid hydroxyl group in pure solvent, a relatively simple procedure is described for the calculation of equi-eluotropic binary solvent systems in liquid-solid chromatography of steroids. This method is compared with the proposals of Snyder and Hara.
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Petrović, S.M., Kolarov, L.A. & Petrović, J.A. Solvent selection in liquid-solid chromatography of steroids. Chromatographia 18, 145–148 (1984). https://doi.org/10.1007/BF02258771
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DOI: https://doi.org/10.1007/BF02258771