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Modifier effects on packed and capillary columns in supercritical fluid chromatography

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Summary

The separation of polar thermally labile solutes is one of the potentially most rewarding fields of SFC application. A presupposition for such applications is, however, mobile phases having relatively high solvent strengths. A promising approach to achieve this is the use of mobile phases consisting of carbon dioxide with a polar additive. In this work, the chromatographic effects of different concentrations of an additive, isopropanol, in carbon dioxide have been studied on capillary and packed columns. A series of antibiotics was used as test substances. Best results were obtained with carbon dioxide/8% isopropanol as mobile phase on a capillary column coated with a cyanopropyl-substituted polysiloxane stationary phase.

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Authors and Affiliations

  1. Department of Analytical Chemistry, University of Stockholm, Arrhenius Laboratory, S-106 91, Stockholm, Sweden

    S. Schmidt & L. G. Blomberg

  2. Lee Scientific, Inc., 84123, Salt Lake City, UT, USA

    E. R. Campbell

Authors
  1. S. Schmidt
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  2. L. G. Blomberg
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  3. E. R. Campbell
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Schmidt, S., Blomberg, L.G. & Campbell, E.R. Modifier effects on packed and capillary columns in supercritical fluid chromatography. Chromatographia 25, 775–780 (1988). https://doi.org/10.1007/BF02262083

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  • DOI: https://doi.org/10.1007/BF02262083

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