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Acute paw oedema formation induced by ATP: Re-evaluation of the mechanisms involved

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Abstract

ATP-induced inflammation was investigated using subplantar injection in the mouse hind paw. The order of efficacy of purinoceptor agonists for inducing paw oedema (30 nmol per paw) was ATP=α,β-methylene ATP=2-methylthio ATP > adenosine > UTP > ADP > AMP. Diadenosine polyphosphates effectively induced paw oedema formation with an order of efficacy of: P1, P4-di(adenosine-5′)tetraphosphate =P1,P5-di(adenosine-5′)-pentaphosphate= P1,P6-di(adenosine-5′) hexaphosphate ≫ ATP=P1,P3-di(adenosine-5′)triphosphate > P1,P2-di(adenosine-5′)pyrophosphate. Systemic administration of P2-purinoceptor antagonists (30–100 μmol/kg), suramin, 4,4′-diisothiocyanatostilbene-2,2′-disulphonate, pyridoxalphosphate-6-azophenyl-2′,4′-disulphonic acid and cibacron blue, reduced the intensity of ATP-induced oedema. At 30 μmol/kg 8-(p-sulfophenyl)theophylline (non-selective adenosine receptor antagonist), 3,7-dimethyl-1,1-propargyl-xanthine (adenosine A2 receptor antagonist), triprolidine (histamine H1 receptor antagonist), ranitidine (histamine H2 receptor antagonist) and ketanserin (5-hydroxytryptamine 5-HT2 receptor antagonist), but neither 8-cyclopentyl-1,3-dipropylxanthine (adenosine A1 receptor antagonist), nor indomethacin (cyclooxygenase inhibitor) inhibited the ATP-induced swelling. Topical (100 nmol per paw), but not systemic (100 μmol/kg) administration of NG-nitro-L-arginine methyl ester (nitric oxide synthase inhibitor) reduced the intensity of the ATP-induced paw oedema. These results show that ATP can induce an inflammatory oedematous reaction and contribute to our understanding of the underlying mechanisms.

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accepted by G. Bowen

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Ziganshina, L.E., Ziganshin, A.U., Hoyle, C.H.V. et al. Acute paw oedema formation induced by ATP: Re-evaluation of the mechanisms involved. Inflamm Res 45, 96–102 (1996). https://doi.org/10.1007/BF02265122

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  • DOI: https://doi.org/10.1007/BF02265122

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