Skip to main content
SpringerLink
Log in

Use of the balanced-cell technique to determine properties of single yeast cells

  • Published:
Journal of Biological Physics Aims and scope Submit manuscript

Abstract

The dielectrophoretic force on single yeast cells in water has been measured by the method of balancing it against the force of gravity. The force expression for the electrode geometry of parallel wires is derived and related to the electrical properties of the cell and its surroundings through the parameter K, the excess relative permittivity. A detailed analysis is given of the two alternate experimental approaches that can be taken using the balance technique. Experimental values of K are determined for various conditions and compared with those predicted from a theoretical model of a yeast cell.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pohl, H. A. 1951. J. Appl. Phys.,22, 869.

    Article  Google Scholar 

  2. Pohl, H. A., and C. E. Plymale. 1960. J. Electrochem. Soc.,107, 390.

    Google Scholar 

  3. Pohl, H. A., and I. Hawk. 1966. Science,152, 647.

    ADS  Google Scholar 

  4. Crane, J. S., and H. A. Pohl. 1968. J. Electrochem. Soc.,115, 584.

    Google Scholar 

  5. Pohl, H. A. (in press). DIELECTROPHORESIS — The Behavior of Matter in Nonuniform Electric Fields, Cambridge University Press, Cambridge, England.

  6. Hawk, I. 1966. “Effects of Nonuniform Electric Fields on Real Dielectrics in Water.” Master’s Thesis, Oklahoma State University.

  7. Feeley, C. M. 1969. “Dielectrophoresis of Solids in Liquids of Differing Dielectric Constant and Conductivity.” Master’s Thesis, Oklahoma State University.

  8. Pohl, H. A., and J. S. Crane. 1971. Biophys. j.,11, 711.

    Google Scholar 

  9. Wiley, K. L. 1970. “A Comparison of Normal and Abnormal Cells Using Dielectrophoresis.” Master’s Thesis, Oklahoma State University.

  10. Chen, C. S., and H. A. Pohl. 1974. Trans. N. Y. Acad. Sci.,238, 176.

    Google Scholar 

  11. Pohl, H. A. 1968. J. Electrochem. Soc.,115, 1c.

    Google Scholar 

  12. von Hippel, A. H. 1954. DIELECTRICS and WAVES. John Wiley and Sons, Inc., New York.

    Google Scholar 

  13. Basore, B. private communication. Oklahoma State University.

  14. Sher, L. D. 1968. Nature, (London),220, 695.

    Google Scholar 

  15. Pohl, H. A., and J. S. Crane. 1972. J. Theo. Biol.,37, 1.

    Google Scholar 

  16. Crane, J. S., and H. A. Pohl. 1972. J. Theo. Biol.,37 15.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physics, Oklahoma State University, 74074, Stillwater, Oklahoma

    Joe S. Crane & Herbert A. Pohl

Authors
  1. Joe S. Crane
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Herbert A. Pohl
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Crane, J.S., Pohl, H.A. Use of the balanced-cell technique to determine properties of single yeast cells. J Biol Phys 5, 49–74 (1977). https://doi.org/10.1007/BF02310090

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02310090

Keywords

Search

Navigation