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Gel filtration and high-performance liquid chromatographic analysis of phosphotungstic acid soluble peptides from blue cheeses

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Summary

The nitrogen fraction from cheese, soluble in phosphotungstic acid has been analyzed by gel permeation and high performance liquid chromatography. Elution profiles of this fraction on Sephadex G-10 show that there are no peptides of molecular weight higher than 700 daltons. Six fractions have been obtained. The first one has the peptides and some amino acids. Fractions II to VI contains mostly free amino acids. Fraction I has been separated into many peaks by HPLC. Six or seven of these peaks are presumed to correspond to peptides.

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Authors and Affiliations

  1. Instituto de Fermentaciones Industriales, CSIC, Juan de la Cierva 3, E-28006, Madrid, Spain

    D. Gonzalez de Llano, M. Ramos & C. Polo

Authors
  1. D. Gonzalez de Llano
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  2. M. Ramos
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  3. C. Polo
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Additional information

A part of this work has been presented in XXI Reunión Bienal de la Socieded Española de Química. Sept. 1986. Santiago de Compostela. España.

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de Llano, D.G., Ramos, M. & Polo, C. Gel filtration and high-performance liquid chromatographic analysis of phosphotungstic acid soluble peptides from blue cheeses. Chromatographia 23, 764–766 (1987). https://doi.org/10.1007/BF02312670

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  • DOI: https://doi.org/10.1007/BF02312670

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