Summary
Resolution of enantiomeric mixtures of DL-amino acids (Nine) using silica gel layers impregnated with (-)-bruncine is reported. The solvent system used was Butanol: Acetic acid: Chloroform (3∶1∶4). The diastereomers were formed and hydrolysed, by dilute HCl spray, on the chromatogram only and the amino acids thus resolved were located by ninhydrin spray. The cross resolution possibilities of enantiomers were also calculated.
This is a preview of subscription content, log in via an institution to check access.
References
IUPAC Tentative Rules for the Nomenclature of Organic Chemistry, Section E, J. Org. Chem.35, 2849 (1970).
M. L. Anson, K. Bailey, J. T. Edsall (Eds.), Advances in Protein Chemistry, Vol.9. Academic Press, N.Y. (1954). pp. 121.
R. Bhushan, R. N. Goyal, A. Agarwal, J. Protein Chemistry,3, 395 (1984).
R. Bhushan, R. N. Goyal, A. Agarwal, Biochemistry Internatl.,11, 477 (1985).
S. P. Srivastava, R. Bhushan, R. S. Chauhan, J. Liquid Chromatography7, 1359 (1984).
R. Bhushan, I. Ali, J. Liquid Chromatography9 (1986) in press.
R. S. Cahn J. Chem. Educ.,41(3), 116 (1964).
B. P. Sleckman, J. Sherma, J. Liquid Chromatogr.,5, 1051 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bhushan, R., Ali, I. TLC resolution of enantiomeric mixtures of amino acids. Chromatographia 23, 141–142 (1987). https://doi.org/10.1007/BF02312891
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02312891