Abstract
Methods previously established for the propagation of cassava plants free from cassava mosaic disease have been applied to Nigerian clones. Meristem tips from diseased plants subjected to heat treatment for not less than 30 days at 35°–38°C were cultured on modified Murashige-Skoog medium. Concentration ranges of benzyladenine in combination with α-naphthalene acetic acid and gibberellic acid were investigated and, at optimal levels, 36% of the meristems regenerated. Regenerants, with callus and shoots only, were rooted with 80% efficiency by sub-culturing following a dip in a hormone rooting powder. All plants raised from heat-treated meristems were free of the disease as judged by visual inspection of the leaves, rooted explants and assay for the suspected pathogenic agent of the disease.
Similar content being viewed by others
References
Adejare GO (1981) Nigerian isolates of cassava latent visus. M Phil thesis, University of London
Beck BDA, Chant SR (1958) Tropical Agriculture 35: 59–64
Berbee FM, Berbee JG, Hildebrandt AC (1973) In Vitro [Abstr] 8: 421
Bock KR, Guthrie EJ (1978) Plant Dis Rep 62: 580–581
Chant SR (1959) Emp J of Exp Agric 27: 55–58
Kaiser WJ, Teemba LR (1979) Plant Dis Rep 63:780–784
Kartha KK, Gamborg OL, Constabel F, Shyluk JP (1974) Plant Sci Lett 2: 107–113
Kartha KK, Gamborg OL (1975) Phytopathology 65: 826–828
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Nair NG, Kartha KK, Gamborg OL (1979) Z Pflanzenphysiol 95: 51–56
Quak F (1972) In: Proc 10th Int Hort Cong 3: 12–25
Shahin EA, Shepard JF (1980) Plant Sci Lett 17: 459–465
Storey MH, Nichols RFW (1938) Ann Appl Biol 25: 790–806
Zeigler RS, Powell LE, Thurston HD (1980) Phytopathology 70: 589–593
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Adejare, G.O., Coutts, R.H.A. Eradication of cassava mosaic disease from Nigerian cassava clones by meristem-tip culture. Plant Cell Tiss Organ Cult 1, 25–32 (1981). https://doi.org/10.1007/BF02318900
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02318900