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Vegetative multiplication of sugarbeet through in vitro culture of inflorescence pieces

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Abstract

In vitro vegetative multiplication of sugarbeet was obtained by culturing of inflorescence explants. Subapical segments or 5-mm-long tips from nine varieties developed axillary shoots (up to 50 per tip) on a medium containing indolebutyric acid (IBA) and benzylaminopurine (BAP). Zeatin was ineffective as cytokinin. Gibberellic acid (GA3) enhanced the process. Such vegetative shoots were subsequently isolated and were each allowed to develop up to 20 supplementary axillary shoots on a multiplication medium containing IBA, BAP, and naphthaleneacetic acid (NAA). Rooting of shoots was obtained in the absence of growth regulators and plants were established.

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Authors and Affiliations

  1. Hormonologie Fondamentale et Appliquée, Institut de Botanique (B22), Université de Liège - Sart Tilman, B-4000, Liège, Belgium

    M. F. Coumans-Gilles, Cl. Kevers, M. Coumans, E. Ceulemans & Th. Gaspar

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  1. M. F. Coumans-Gilles
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  2. Cl. Kevers
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  3. M. Coumans
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  4. E. Ceulemans
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  5. Th. Gaspar
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Coumans-Gilles, M.F., Kevers, C., Coumans, M. et al. Vegetative multiplication of sugarbeet through in vitro culture of inflorescence pieces. Plant Cell Tiss Organ Cult 1, 93–101 (1981). https://doi.org/10.1007/BF02318908

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  • DOI: https://doi.org/10.1007/BF02318908

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