Abstract
Effects of Cd2+ on growth and Cd-binding complex formation in roots were examined with various seedlings of mono- and dicotyledonous plants. Maize, oat, barley and rice exhibited the greater tolerance to Cd2+ (100 μM) than did azuki bean, cucumber, lettuce, pea, radish, sesame and tomato (10–30 μM). Azuki bean was the most sensitive to Cd2+ (<10 μM). Under these Cd-treatments, cereal roots accumulated Cd2+ in the cytoplasmic fractions and transported Cd2+ into the same fractions of shoot tissues, to larger extents than did dicotyledonous roots. Cereal roots synthesized a Cd-binding complex containing phytochelatins in the cytoplasmic fractions, depending upon Cd2+ concentrations applied (30–100 μM). Such a complex was not detected from the same fractions of dicotyledonous roots treated with Cd2+. These results suggest that the Cd-binding complex formation has an important role in the tolerance of cereal roots against Cd2+.
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Abbreviations
- GPC:
-
gel-permeation chromatography
- GSH:
-
glutathione
- HPLC:
-
high-performance liquid chromatography
- PC:
-
phytochelatin
- PCMB:
-
p-chloromercuribenzoate
- TFA:
-
trifluoroacetic acid
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Inouhe, M., Ninomiya, S., Tohoyama, H. et al. Different characteristics of roots in the cadmium-tolerance and Cd-binding complex formation between mono- and dicotyledonous plants. J. Plant Res. 107, 201–207 (1994). https://doi.org/10.1007/BF02344245
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DOI: https://doi.org/10.1007/BF02344245