Summary
The hemicellulase separated from birchwood by steaming and water extraction comprised mainly of acetyl- and 4-O-methyglucurono-substituted xylo-oligomers. The liberation of the acidic side groups affected the rate and yield of the enzymatic hydrolysis of the xylo-oligomers. The hemicellulase ofTrichoderma reesei was superior to that ofAspergillus awamori both with respect to side group cleavage and xylose yield in hydrolysis.
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References
Biely P, Puls J, Schneider H (1985) Acetyl xylan esterases in fungal cellulolytic systems, FEBS Lett 186, 1:80–84
Biely P, MacKenzie CR, Puls J, Schneider H (1985) Acetyl xylan esterases — A novel class of microbial enzymes involved in the degradation of hemicellulose, International Symposium on Wood and Pulping Chemistry, Vancouver, Aug 26–30, Preprint Book, p 93
Dekker RFH (1983) Bioconversion of Hemicellulose: Aspects of hemicellulose production byTrichoderma reesei QM 9414 and enzymatic saccharification of hemicellulose. Biotechnol Bioeng 30:1127–1146
Frederick MM, Kiang C-H, Frederick JR, Reilly PJ (1985) Purification and characterization of endo-xylanases fromAspergillus niger. I. Two isoenzymes active on xylan backbones near branch points. Biotechnol Bioeng 27:525–532
Maloney MT, Chapman TW, Baker AJ (1985) Dilute acid hydrolysis of paper birch: kinetic studies of xylan and acetylgroup hydrolysis. Biotechnol Bioeng 27:355–361
Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380
Nummi M, Perrin J-M, Niku-Paavola M-L, Enari T-M (1985) Measurement of xylanase activity with insoluble xylan substrate. Biochem J 226:617–620
Puls J, Sinner M, Dietrichs H-H (1978) Trägergebundene xylanolytische Enzyme. I. Wirkungsweise xylanolytischer Enzyme. Stärke 30, 9:294–299
Puls J, Poutanen K, Körner H-U, Viikari L (1985) Biotechnical utilization of wood carbohydrates after steaming pretreatment. Appl Microbiol Biotechnol 22:416–423
Saddler JN, Yu EKC, Mes-Hartree M, Levitin N, Brownell H (1983) Utilization of enzymatically hydrolyzed wood hemicelluloses by micro-organisms for production of liquid fuels. Appl Environ Microbiol 45 (1):153–160
Sinner M, Simatupang MH, Dietrichs HH (1975) Automated quantitative analysis of wood carbohydrates by borate complex ion exchange chromatography. Wood Sci Technol 9:307–322
Sinner M, Puls J (1978) Non-corrosive dye reagent for detection of reducing sugars in borate complex ion-exchange chromatography. J Chromatogr 156:197–204
Somogyi M (1952) Determination of blood sugar. J Biol Chem 195:19–23
Timell TE (1962) Enzymatic hydrolysis of a 4-O-methylglucuronoxylan from the wood of white birch. Svensk Papperstidn (65) 11:435–447
Timell TE (1967) Recent progress in the chemistry of wood hemicelluloses. Wood Sci Technol 1:45–70
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Poutanen, K., Puls, J. & Linko, M. The hydrolysis of steamed birchwood hemicellulose by enzymes produced byTrichoderma reesei andAspergillus awamori . Appl Microbiol Biotechnol 23, 487–490 (1986). https://doi.org/10.1007/BF02346065
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DOI: https://doi.org/10.1007/BF02346065