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A combination of sister chromatid differential staining and giemsa banding

  • Specialia Biologica
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Summary

We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists.

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  8. Supported in part by National Science Foundation Research Grant No. GB-41296 and Research Grant No. VC-21 from the American Cancer Society and NIH Training Grant CA-5047 from the National Cancer Institute. We wish to thank ProfessorT. C. Hsu and Dr.F. E. Arrighi for their encouragement and helpful discussion.

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Author notes

  1. A. Lusby

    Present address: Graduate School of Biomedical Sciences, The University of Texas, 77025, Houston, Texas, USA

Authors and Affiliations

  1. Department of Biology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 77025, Houston, Texas, USA

    S. Pathak, A. D. Stock & A. Lusby

  2. Graduate School of Biomedical Sciences, The University of Texas, 77025, Houston, Texas, USA

    S. Pathak & A. D. Stock

Authors
  1. S. Pathak
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  2. A. D. Stock
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  3. A. Lusby
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Pathak, S., Stock, A.D. & Lusby, A. A combination of sister chromatid differential staining and giemsa banding. Experientia 31, 916–918 (1975). https://doi.org/10.1007/BF02358850

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  • DOI: https://doi.org/10.1007/BF02358850

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