Résumé
La comparaison des granules ou des protéines de granules desBaculovirus dePieris brassicae, Pygaera anastomosis, Hyphantria cunea, Carpocapsa pomonella, Mythimna unipuncta, Mamestra oleracea a été entreprise par les techniques d'électrophorèse, d'agglutination, d'immunofluorescence et de précipitation en gel. L'électrophorèse fait apparaître l'étroite ressemblance qui existe entre les protéines des six sortes de corps d'inclusion. L'analyse immunochimique montre que chaque granule est bien distinct de tous les autres; elle peut servir de base à l'identification des divers virus.
Summary
The proteins ofBaculovirus capsules ofPieris brassicae, Pygaera anastomosis, Hyphantria cunea, Mamestra oleracea, Mythimna unipuncta andCarpocapsa pomonella are studied and compared.
There is no significant difference in the composition of electrophoregrams of proteins extracted from capsules of six virus strains by means of a thioglycolate buffer, pH 10,5. Two chief zones (G and F bands) are identified in every one of these strains. Mobility of proteins (C proteins) contained in the G intermediate band is slightly identical, only C proteins ofPieris brassicae andPygaera anastomosis can be differentiated according to a slight difference between electrophoretic mobility.
Agglutination and immunofluorescence techniques used for suspensions and smears of granules enable to recognize granules ofC. pomonella andM. unipuncta among the six types of granules.
By the immunodiffusion technique, it is revealed that F antigens occurs in all strains and C antigens in five ones. In addition to these common antigens, there are specific antigens respectively named TPa, TPb, J, U, P antigens forP. anastomosis, P. brassicae, H. cunea, M. unipuncta andC. pomonella granules.
Capsules taken in Hungary and France from larvae ofC. pomonella could not be differentiated by the techniques used.
This work emphasizes both characters common to the group of viruses studied and differences sufficient for identifying every one of types studied.
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Avec la collaboration technique de LilianeCroizier.
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Croizier, G., Meynadier, G. Les protéines des corps d'inclusion desBaculovirus . Entomophaga 18, 259–269 (1973). https://doi.org/10.1007/BF02371473
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DOI: https://doi.org/10.1007/BF02371473