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Optimization of batch fermentation conditions for dextran production

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Abstract

The nutrient medium (containing sucrose, yeast extract and K2HPO4), temperature and initial pH conditions were optimised for batch dextran production in shake flask fermentations using a strain of Leuconostoc mesenteroides NRRL B 512 (F). A 25−1 fractional factorial central composite experimental design was attempted. Multistage Monte Carlo optimization program was used to maximize the multiple regression equation obtained. The optimal values of tested variables for maximal dextran production were found to be: sucrose, 300 g/l; yeast extract, 10 g/l; K2HPO4, 30 g/l; temperature, 23°C and initial pH 8.3 with a predicted dextran yield of 154 g/l.

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Abbreviations

Z i :

Uncoded value ofith independent variable

Z *i :

Uncoded value ofith independent variable at centre point

ΔZ i :

Step change value

X i :

Coded value ofith Independent variable

Y j :

Observed dextran yield ofjth experiment (g/L)

\(\hat Y_j \) :

Predicted dextran yield ofjth experiment (g/L)

RSM:

Response surface methology

MSMCO:

Multistage Monte Carlo optimization

R2 :

Coefficient of determination

R:

Multiple correlation coefficient

S 2r :

Variance explained by regression model

S 2e :

Residual variance

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Authors and Affiliations

  1. Biotechnology Research Center, Department of Chemical Engineering, Indian Institute of Technology, 600 036, Madras, India

    R. S. Karthikeyan, S. K. Rakshit & A. Baradarajan

Authors
  1. R. S. Karthikeyan
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  2. S. K. Rakshit
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  3. A. Baradarajan
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Karthikeyan, R.S., Rakshit, S.K. & Baradarajan, A. Optimization of batch fermentation conditions for dextran production. Bioprocess Engineering 15, 247–251 (1996). https://doi.org/10.1007/BF02391585

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