Skip to main content
SpringerLink
Log in

Molybdate metabolism inAspergillus nidulans

II. Mutations affecting phosphatase activity or galactose utilization

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Certain mutants ofAspergillus nidulans defective in galactose utilization are resistant to molybdate. However, mutational loss of galactokinase and/or galactose-1-phosphate uridyl transferase does not lead to molybdate resistance. Another class of molybdate resistant mutants utilizes galactose faster than the wild type and is hypersensitive to acriflavine. Certain mutations affecting phosphatase activity lead to resistance or hypersensitivity to molybdate. The pattern of molybdate tolerance among phosphatase mutants suggests that loss of an acid phosphatase (phosphatase IV) is associated with molybdate resistance while enhanced levels of this phosphatase produce hypersensitivity. It is concluded that a phosphorylated entity and a carbohydrate-containing compound synthesized from glucose-1-phosphate, uridine diphosphoglucose, or uridine diphosphogalactose participate in molybdate metabolism inA. nidulans and are possibly involved in molybdate uptake.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arst, H. N., Jr.: Genetic analysis of the first steps of sulphate metabolism inAspergillus nidulans. Nature (Lond.)219, 268–270 (1968).

    Article  CAS  Google Scholar 

  • —, MacDonald, D. W., Cove, D. J.: Molybdate metabolism inAspergillus nidulans. I. Mutations affecting nitrate reductase and/or xanthine dehydrogenase. Molec. Gen. Genetics108, 129–145 (1970).

    CAS  Google Scholar 

  • Caprioli, R., Rittenberg, D.: Quantitative aspects of the origin of pentose inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)60, 1379–1382 (1968a).

    Article  CAS  Google Scholar 

  • ——: On the utilization of D-fructose for pentose synthesis inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)61, 1422–1427 (1968b).

    Article  CAS  Google Scholar 

  • ——: Pentose synthesis inEscherichia coli. Biochemistry (Wash.)8, 3375–3384 (1969).

    CAS  Google Scholar 

  • Cove, D. J.: The induction and repression of nitrate reductase in the fungusAspergillus nidulans. Biochim. biophys. Acta (Amst.)113, 51–56 (1966).

    CAS  Google Scholar 

  • —, Pateman, J. A.: Independently segregating loci concerned with nitrate reductase activity inAspergillus nidulans. Nature (Lond.)198, 262–263 (1963).

    Article  CAS  Google Scholar 

  • Dorn, G. L.: Genetic analysis of the phosphatases inAspergillus nidulans. Genet. Res.6, 13–26 (1965a).

    Google Scholar 

  • —: Phosphatase mutants inAspergillus nidulans. Science150, 1183–1184 (1965b).

    PubMed  CAS  Google Scholar 

  • —: Purification of two alkaline phosphatases fromAspergillus nidulans. Biochim. biophys. Acta (Amst.)132, 190–193 (1967a).

    CAS  Google Scholar 

  • —: A revised map of the eight linkage groups ofAspergillus nidulans. Genetics56, 619–631 (1967b).

    PubMed  CAS  Google Scholar 

  • —: Purification and characterization of phosphatase I fromAspergillus nidulans. J. biol. Chem.243, 3500–3506 (1968).

    PubMed  CAS  Google Scholar 

  • —, Riviera, W.: Kinetics of fungal growth and phosphatase formation inAspergillus nidulans. J. Bact.92, 1618–1622 (1966).

    PubMed  CAS  Google Scholar 

  • McCully, K. S., Forbes, E.: The use ofp-fluorophenylalanine with “master strains” ofAspergillus nidulans for assigning genes to linkage groups. Genet. Res.6, 352–359 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Nagel de Zwaig, R., Luria, S. E.: Genetics and physiology of colicin-tolerant mutants ofEscherichia coli. J. Bact.94, 1112–1123 (1967).

    PubMed  CAS  Google Scholar 

  • Nakamura, H.: Acriflavine-binding capacity ofEscherichia coli in relation to acriflavine sensitivity and metabolic activity. J. Bact.92, 1447–1452 (1966).

    PubMed  CAS  Google Scholar 

  • —: Genetic determination of resistance to acriflavine, phenethyl alcohol, and sodium dodecyl sulfate inEscherichia coli. J. Bact.96, 987–996 (1968a).

    PubMed  CAS  Google Scholar 

  • —: Acriflavine resistance inEscherichia coli: The role of the cell membrane. Memoirs of the Konan University. Science Series11, 43–62 (1968b).

    Google Scholar 

  • Pateman, J. A., Cove, D. J., Rever, B. M., Roberts, D. B.: A common co-factor for nitrate reductase and xanthine dehydrogenase which also regulates the synthesis of nitrate reductase. Nature (Lond.)201, 58–60 (1964).

    Article  CAS  Google Scholar 

  • —, Rever, B. M., Cove, D. J.: Genetic and biochemical studies of nitrate reduction inAspergillus nidulans. Biochem. J.104, 103–111 (1967).

    PubMed  CAS  Google Scholar 

  • Pontecorvo, G., Roper, J. A., Hemmons, L. M., Macdonald, K. D., Bufton, A. W. J.: The genetics ofAspergillus nidulans. Advanc. Genet.5, 141–238 (1953).

    Article  CAS  Google Scholar 

  • Roberts, C. F.: The genetic analysis of carbohydrate utilization inAspergillus nidulans. J. gen. Microbiol.31, 45–58 (1963a).

    PubMed  CAS  Google Scholar 

  • —: The adaptive metabolism of D-galactose inAspergillus nidulans. J. gen. Microbiol.31, 285–295 (1963b).

    PubMed  CAS  Google Scholar 

  • —: Enzyme lesions in galactose non-utilising mutants ofAspergillus nidulans. Biochim. biophys. Acta (Amst.)201, 267–283 (1970).

    CAS  Google Scholar 

  • Scazzocchio, C.: Studies on the genetic control of purine oxidation inAspergillus nidulans. Ph. D. thesis, University of Cambridge (1966).

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, University of Cambridge, CB4 1XH, Cambridge, England

    H. N. Arst Jr. & D. J. Cove

Authors
  1. H. N. Arst Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. J. Cove
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arst, H.N., Cove, D.J. Molybdate metabolism inAspergillus nidulans . Molec. Gen. Genet. 108, 146–153 (1970). https://doi.org/10.1007/BF02430520

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02430520

Keywords

Search

Navigation