Abstract
The starch-degrading yeastCandida tsukubaensis CBS 6389 secreted amylase at high activity when grown in a medium containing soluble starch. The extracellular α-amylase activity was very low. The major amylase component was purified by DEAE-Sephadex A-50 chromatography and Ultrogel AcA 44 gel filtration and characterized as a glucoamylase. The enzyme proved to be a glycoprotein with a molecular weight of 56000. The glucoamylase had a temperature optimum at 55°C and displayed highest activity in a pH range of 2.4–4.8. Acarbose strongly inhibited the purified glucoamylase. Debranching activity was present as demonstrated by the release of glucose from pullulan.
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De Mot, R., Van Oudenduck, E. & Verachtert, H. Purification and characterization of an extracellular glucoamylase from the yeastCandida tsukubaensis CBS 6389. Antonie van Leeuwenhoek 51, 275–287 (1985). https://doi.org/10.1007/BF02439937
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DOI: https://doi.org/10.1007/BF02439937