Summary
Modifications to secondary structure and fibril formation caused by multiple acetylHmb backbone amide protection of Alzheimer's disease Aβ(1–40) were investigated using circular dichroism spectroscopy and electron microscopy. Penta(acetylHmb) Aβ(1–40) was observed to have a reduced ability to form α-helix and β-sheet structures under the same solution conditions as the native peptide, with α-helical propensity being reduced more significantly than β-sheet propensity. Further, acetylHmb backbone protection was found to alter Aβ(1–40) interaction with SDS-micelles by preventing α-helix formation. Aβ fibril formation, a characteristic property of this peptide, was also not observed for penta(acetylHmb) Aβ(1–40).
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Clippingdale, A.B., He, WL., Macris, M. et al. Secondary structural modifications of Aβ(1–40) induced by multiple 2-acetoxy-4-methoxybenzyl (acetylHmb) protection. Lett Pept Sci 6, 289–293 (1999). https://doi.org/10.1007/BF02443424
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DOI: https://doi.org/10.1007/BF02443424