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An automatic multiple column apparatus for analytical zone electrophoresis

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Abstract

A multiple column analytical zone electrophoresis apparatus is described. Twelve columns 0·7×15 cm packed with acetylated cellulose powder are arranged radially around 2 circular electrode vessels. The columns are isolated from the vessels by semi-permeable plugs. At the end of the run of the columns are discharged in sequence through an ultraviolet photometer by means of an automatically operated rotary cock. The apparatus is used in conjunction with an automatic integrating and printing mechanism.

Résumé

L'auteur décrit un appareil à plusieurs colonnes pour l'électrophorèse analytique à zones. 12 colonnes de 0,7 à 15 cm, remplies de pourdre de cellulose acétylée, sont disposées en étoile autour de 2 boîtes à électrodes rondes. Les colonnes sont isolées à l'égard des boîtes par des bouchons semi-perméables. A la fin d'un procédé d'électrophorèse, les colonnes seront déchargées l'une après l'autre à travers un photomètre u.-v., à l'aide d'un robinet rotatoire automatique. L'appareil est utilisé de concert avec un mécanisme automatique d'intégration et d'impression.

Zusammenfassung

Es wird eine Appratur für die Zonenelektrophorese auf mehreren Säulen beschrieben. Zwölf Säulen zu je 0,7 mal 15 cm werden mit Azetyl-Zellulose-Pulver gestopft und kreisförmig um zwei runde Elektroden-Gefäße aufgestellt. Die Säulen sind gegen das Gefäß durch semipermeable Stecker isoliert. Am Ende eines Laufes werden die Säulen nacheinander durch ein Ultraviolett-Photometer mit Hilfe eines automatisch betriebenen rotierenden Hannes entleert. Der Apparat arbeitet in Verbindung mit einem automatisch integrierenden Aufzeichnungsgerät.

Резюме

Описывается аппаратура для зонального электрофореза на нескольких колоннах. 12 колонн размером по 0,7×15см заполняются ацетило-целлюлозным порошком, и устанавливаются по окружности вокруг двух круглых электродных сосудов. Колонны изолированы от сосудов посредством полупроницаемых иітепселей. На конце потока колонны поочередно опорожняются ультрафиолетовым фотометром с помощью врацающегося крана с автоматическим управлением. Аппарат работает в соединении с автоматическим интегрирующим регистрационным прибором.

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Authors and Affiliations

  1. Baker Medical Research Institute, Victoria, Australia

    C. C. Curtain

Authors
  1. C. C. Curtain
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Additional information

Part of the expenses of this investigation were defrayed by a grant from the National Health and Medical Research Council, Canberra, Australia.

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Curtain, C.C. An automatic multiple column apparatus for analytical zone electrophoresis. Med. Electron. Biol. Engng 1, 227–235 (1963). https://doi.org/10.1007/BF02475891

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  • DOI: https://doi.org/10.1007/BF02475891

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