Skip to main content
SpringerLink
Log in

Capillary electrochromatography with 1.5 μm ODS-modified non-porous silica spheres

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

The particle realization of electrochromatography on capillaries packed with 1.5 μm ODS-modified non-porous silica spheres is demonstrated. In order to realize stable separation conditions it is crucial to add sodium dodecylsulphate (SDS) to the mobile phase and to pressurize both buffer vials at 10 bar. The presence of SDS stabilizes the current and makes the electro-osmotic flow in the packing more uniform so that no air bubbles are generated at high field strengths. The capillary columns are extremely efficient and on a 24 cm long column about 120,000 plates can be generated (a reduced plate height of about 1.3). The columns are very stable and no loss in efficiency was found after using a column continuously for two months.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. F. K. Huber, Ber. Bunsenges. Physik. Chem.77, 179 (1973).

    CAS  Google Scholar 

  2. J. H. Knox, M. Saleem, J. Chromatogr. Sci.7, 614 (1969).

    CAS  Google Scholar 

  3. H. Giesche, K. K. Unger, U. Esser, B. Eray, U. Trudinger, J. N. Kinkel J. Chromatogr.465, 39 (1989).

    Article  CAS  Google Scholar 

  4. V. Pretorius, B. J. Hopkins, J. D. Schieke, J. Chromatogr.99, 23 (1974).

    Article  CAS  Google Scholar 

  5. J. H. Knox, I. H. Grant Chromatographia24, 135 (1987).

    CAS  Google Scholar 

  6. J. H. Knox, I. H. Grant, Chromatographia32, 317 (1991).

    Article  CAS  Google Scholar 

  7. N. W. Smith, M. B. Evans, Chromatographia38, 649 (1994).

    Article  CAS  Google Scholar 

  8. H. Rebscher, U. Pyell, Chromatographia38, 737 (1994).

    Article  CAS  Google Scholar 

  9. R. J. Boughtflower, T. Underwood, C. J. Paterson, Chromatographia40, 329 (1995).

    Article  CAS  Google Scholar 

  10. S. E. van den Bosch, H. Heemstra, J. C. Kraak, H. Poppe, J. Chromatogr. A755, 165 (1996).

    Article  Google Scholar 

  11. R. J. Boughtflower, T. Underwood, J. Maddin, Chromatographia41, 398 (1995).

    Article  CAS  Google Scholar 

  12. C. Yan, R. Dadoo, H. Zhao, R. N. Zare, D. J. Rakestraw, Anal. Chem.67, 2026 (1995).

    Article  CAS  Google Scholar 

  13. M. M. Dittmann, K. Wienand, F. Bek, G. P. Rozing, LC-GC13, (10) 800 (1995).

    CAS  Google Scholar 

  14. M. M. Dittman, G. P. Rozing, J. Chromatogr. A744, 63. (1996).

    Article  Google Scholar 

  15. F. Lelievre, C. Yan, R. N. Zare, P. Garei, J. Chromatogr. A723, 145, (1996).

    Article  Google Scholar 

  16. M. T. Dulay, C. Yan, D. J. Rakestraw, R. N. Zare, J. Chromatogr. A725, 361 (1996).

    Article  CAS  Google Scholar 

  17. H. Yamamoto, J. Baumann, F. Erni, J. Chromatogr.593, 313 (1992).

    Article  CAS  Google Scholar 

  18. J. H. Knox, G. Laird, J. Chromatogr.122, 17 (1976).

    Article  CAS  Google Scholar 

  19. J. C. Kraak, K. M. Jonker, J. F. K. Huber, J. Chromatogr.142, 671 (1977).

    Article  CAS  Google Scholar 

  20. E. A. G. Aniansson, S. N. Wall, M. Almgren, H. Hoffmann, I. Kielmann, W. Ulbricht, R. Zana, J. Lang, C. Tondre, J. Phys. Chem.80, 905 (1976).

    Article  CAS  Google Scholar 

  21. S. Terabe, K. Otsuka, K. Ichikawa, A. Tsuchiya, T. Ando, Anal. Chem.56, 111 (1984).

    Article  CAS  Google Scholar 

  22. P. Delahay, Double Layer and Electrode Kinetics, Wiley-Interscience, New York, 1965.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Amsterdam Institute for Molecular Studies, Laboratory for Analytical Chemistry, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV, Amsterdam, The Netherlands

    R. M. Seifar, W. Th. Kok, J. C. Kraak & H. Poppe

Authors
  1. R. M. Seifar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Th. Kok
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. C. Kraak
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Poppe
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Seifar, R.M., Kok, W.T., Kraak, J.C. et al. Capillary electrochromatography with 1.5 μm ODS-modified non-porous silica spheres. Chromatographia 46, 131–136 (1997). https://doi.org/10.1007/BF02495322

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02495322

Key Words

Search

Navigation