Skip to main content
Log in

High-resolution HKG-banding in maize mitotic chromosomes

  • Original Articles
  • Published:
Journal of Plant Research Aims and scope Submit manuscript

Abstract

Root-tip maize chromosomes (2n=20) were prepared by high-resolution procedures after ethidium bromide/colchicine synchronization. Using HKG-banding (HCl−KOH-Giemsa), that shows both centromeric and intercalary heterochromatin, the banding pattern of the elongated-chromosomes showed one to nine well-resolved dark bands. Differences of HKG-banding pattern of elongated and compacted chromosomes were performed by image analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Belyayev, A. 1993. Banding of G- or R-type in prophase/prometaphase in the M-chromosome ofVicia faba L. Caryologia46: 301–307.

    Google Scholar 

  • Carlson, W. R. 1988. The cytogenetics of corn.In G.F. Sprague and J.W. Dudley, eds., Corn and Corn Improvement, ASA Monograph, vol. 18, American Society of Agronomy, Madison, pp. 259–344.

    Google Scholar 

  • Carvalho, C.R. andSaraiva, L.S. 1993. A new heterochromatin banding pattern revealed by modified HKG-banding technique for maize chromosomes. Heredity,70: 515–519.

    Google Scholar 

  • Carvalho, C.R. 1995. Desenvolvimento de Tecnologia Citogenética em Milho (Zea mays L.). D.Sc. Thesis, Universidade Federal de Viçosa, MG., Brazil.

  • Kakeda, K., Yamagata, H., Fukui, K., Ohno, M., Fukui, K., Wei, Z.Z. andZhu, F.S. 1990. High resolution bands in maize chromosomes by G-banding methods. Theor. Appl. Genet.80: 265–272.

    Article  Google Scholar 

  • Murata, M. andOrton, T.J. 1984. G-band-like differentiation in mitotic prometaphase chromosomes of celery. J. Heredity75: 225–228.

    Google Scholar 

  • Rasband, W. 1994. NIH Image 1.55. A public domain software for image analysis, written at the US National Institutes of Health and available from the Internet byanonymous ftp from zippy.nimh.nih.gov

  • Shang, M.X., Jackson, R.C. andNguyen H.T. 1988. Heterochromatin diversity and chromosome morphology in wheats analyzed by the HKG banding technique. Genome30: 956–965.

    Google Scholar 

  • Spowart, G. 1994. Mitotic metaphase chromosome preparation from peripheral blood for high-resolution.In J.R. Gosden, ed., Chromosome Analysis Protocols, vol. 29, Humana Press, New Jersey, pp. 1–10.

    Chapter  Google Scholar 

  • Sumner, A.T. 1989. Chromosome banding.In A. Lacey, ed., Light Microscopy in Biology—a Practical Approach, IRL Press, Oxford, pp. 279–314.

    Google Scholar 

  • Sumner, A.T. 1990. Chromosome Banding. Unwin Hyman, London.

    Google Scholar 

  • Verma, R.S. andBabu, A. 1995. Human Chromosomes-Principles and Techniques. 2d ed., McGraw-Hill, New York.

    Google Scholar 

  • Yang, X. andZhang Z. 1988.In situ induction of G-bands and macrocoils in plant chromosomes. Hereditas109: 45–51.

    Google Scholar 

  • Zhu, F., Wei, J., Fu, J. and Li, L. 1986. High resolution banding in chromosomes ofTriticum monococum. Proc. 1st Int. Symp. chromosome Eng. Plants, Xian, 226–227.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

de Carvalho, C.R., Saraiva, L.S. High-resolution HKG-banding in maize mitotic chromosomes. J. Plant Res. 110, 417–420 (1997). https://doi.org/10.1007/BF02506801

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02506801

Key words

Navigation